Approaches to quantifying hepatitis B virus covalently closed circular DNA

被引：13

作者：

Zhang, Henrik ^{[1
,2
]}

Tu, Thomas ^{[1
,2
,3
]}

机构：

[1] Univ Sydney, Westmead Clin Sch, Storr Liver Ctr, 176 Hawkesbury Rd, Westmead, NSW 2145, Australia

[2] Univ Sydney, Westmead Inst Med Res, Fac Med & Hlth, 176 Hawkesbury Rd, Westmead, NSW 2145, Australia

[3] Univ Sydney, Westmead Hosp, Marie Bashir Inst Infect Dis & Biosecur, Ctr Infect Dis & Microbiol, Westmead, NSW, Australia

来源：

CLINICAL AND MOLECULAR HEPATOLOGY | 2022年 / 28卷 / 02期

基金：

英国医学研究理事会;

关键词：

Liver; Immunoenzyme techniques; Polymerase chain reaction; Blotting; Southern; Genome; Viral; CORE-RELATED ANTIGEN; P-GENE-PRODUCT; SURFACE-ANTIGEN; HBV DNA; ADEFOVIR DIPIVOXIL; NATURAL-HISTORY; CELL-LINE; HALF-LIFE; CCC DNA; RNA;

D O I：

10.3350/cmh.2021.0283

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Chronic hepatitis B is a major cause of liver disease worldwide and is currently incurable. Hepatitis B virus (HBV) covalently closed circular (ccc) DNA is a key form of the virus responsible for its persistence and is the transcriptional template for all viral transcripts. The field is focussed on methods to clear HBV cccDNA but this been limited by technical difficulties in its quantification due to: identical sequence to other forms of HBV DNA; low copy number per cell; and high resistance to denaturation by heat, leading to difficulty using polymerase chain reaction or hybridization methods for detection. A number of assays have been developed in order to overcome these hurdles either directly or detecting cccDNA levels indirectly via its transcriptional products. In this review, we summarize the approaches to cccDNA quantification that are currently used, and outline key open questions in the cccDNA biology field which remain to be answered due to the limitations of current methods.

引用

页码：135 / 149

页数：15

共 108 条

[1] Half-life of the duck hepatitis B virus covalently closed circular DNA pool in vivo following inhibition of viral replication [J].