Molecular Signature in Human Cumulus Cells Related to Embryonic Developmental Potential

被引：16

作者：

Xu, Xiaofeng ^{[1
,2
]}

Chen, Dawei ^{[1
,2
]}

Zhang, Zhiguo ^{[1
,2
]}

Wei, Zhaolian ^{[1
,2
]}

Cao, Yunxia ^{[1
,2
]}

机构：

[1] Anhui Med Univ, Affiliated Hosp 1, Reprod Med Ctr, Hefei 230022, Anhui, Peoples R China

[2] Anhui Med Univ, Affiliated Hosp 1, Inst Reprod Genet, Hefei 230022, Anhui, Peoples R China

来源：

REPRODUCTIVE SCIENCES | 2015年 / 22卷 / 02期

关键词：

cumulus cells; COC; gene expression; oocyte quality; developmental potential; DIFFERENTIAL GENE-EXPRESSION; OOCYTE COMPLEX; ADAMTS METALLOPROTEASES; BLASTOCYST DEVELOPMENT; EXTRACELLULAR-MATRIX; IN-VITRO; FERTILIZATION; MATURATION; OVULATION; VERSICAN;

D O I：

10.1177/1933719114536471

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Identification of criteria for embryo quality is required to improve the clinical outcome of in vitro fertilization. The aim of this study was to determine the gene expression profile of cumulus cells (CC) surrounding the oocyte as biomarkers for embryonic developmental potential. CCs from single oocytes were analysed using DNA microarrays. Gene expression profiles of CC surrounding the oocyte associated with good embryonic quality were analyzed. We observed that CCs issued from oocytes that developed into embryos with a good morphology had significantly different gene expression profile from those with bad morphology. These results were confirmed by quantitative RT-PCR. The gene expression profiling of human CC correlates with embryo potential. Our findings suggest anon-invasive approach, offering a new potential strategy for competent embryo selection.

引用

页码：173 / 180

页数：8

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