Long Non-Coding RNA GATA3-AS1 Promotes 5-Fluorouracil Resistance of Ovarian Cancer via Mediating miR-6771-3p/SOX4 Target Axis

被引：1
作者：
Hao, Yafei [1 ]
Li, Changzhou [2 ]
Zhao, Teng [3 ]
Liu, Hansheng [1 ]
机构：
[1] Taian City Cent Hosp, Dept Gynecol, Tai An 271000, Shandong, Peoples R China
[2] Taian City Cent Hosp, Reprod Med Ctr, Tai An 271000, Shandong, Peoples R China
[3] Ningyang Cty Peoples Hosp, Dept Gynecol, Tai An 271400, Shandong, Peoples R China
来源：
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING | 2021年 / 11卷 / 06期
关键词：
GATA3-AS1; Ovarian Cancer; 5-Fluorouracil Resistance; miR-6771-3p; SOX4; TUMOR-GROWTH; LNCRNAS;
D O I：
10.1166/jbt.2021.2666
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Ovarian cancer (OC) ranks as the 5th highest cause of cancer-related deaths worldwide. Long non-coding RNAs (lncRNAs) exert significant effects on chemotherapy resistance. The effects of lncRNA GATA3-AS1 on the 5-Fluorouracil (5-FU) resistance in ovarian carcinoma were explored in the current study. The results showed that GATA3-AS1 was highly expressed in OC tissues and 5-FU resistant OC cells. Moreover, GATA3-AS1 knockdown reduced 50% inhibitory concentration (IC50) of 5-Fluorouracil and promoted cell apoptosis, while GATA3-AS1-overexpression showed the opposite effect. In vivo experiment and murine xenograft assay indicated GATA3-AS1 knockdown inhibited neoplasm growth, promoted cell apoptosis, and altered the expression level of apoptosis-associated proteins. GATA3-AS1 promoted SOX4 expression, a well-known transcription factor regulating apoptosis, via targeting miR-6771-3p. In summary, our findings suggested GATA3-AS1 was associated with OC 5-Fluorouracil via regulating miR-6771-3p/SOX4, providing novel insights into OC chemoresistance.
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页码：1099 / 1107
页数：9
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