Ceramic cells for high pressure NMR spectroscopy of proteins

被引：28

作者：

Erlach, Markus Beck ^{[1
]}

Munte, Claudia E. ^{[1
]}

Kremer, Werner ^{[1
]}

Hartl, Rainer ^{[1
]}

Rochelt, Doerte ^{[1
]}

Niesner, Dieter ^{[1
]}

Kalbitzer, Hans Robert ^{[1
]}

机构：

[1] Univ Regensburg, Dept Biophys, D-93040 Regensburg, Germany

来源：

JOURNAL OF MAGNETIC RESONANCE | 2010年 / 204卷 / 02期

关键词：

High pressure; NMR spectroscopy; Ceramic cell; Proteins; NUCLEAR-MAGNETIC-RESONANCE; HUMAN PRION PROTEIN; HIGH-RESOLUTION; STATES;

D O I：

10.1016/j.jmr.2010.02.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Application of high pressure to biological macromolecules can be used to find new structural states with a smaller specific volume of the system. High pressure NMR spectroscopy is a most promising analytical tool for the study of these states at atomic resolution. High pressure quartz cells are difficult to handle, high quality sapphire high pressure cells are difficult to obtain commercially. In this work, we describe the use of high pressure ceramic cells produced from yttrium stabilized ZrO2 that are capable of resisting pressures up to 200 MPa. Since the new cells should also be usable in the easily damageable cryoprobes a completely new autoclave for these cells has been constructed, including an improved method for pressure transmission, an integrated safety jacket, a displacement body, and a fast self-closing emergency valve. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：196 / 199

页数：4

共 32 条

[1] Highly fluctuating protein structures revealed by variable-pressure nuclear magnetic resonance [J].