Single-cell detection by cavity ring-down spectroscopy

被引：66

作者：

Tarsa, PB ^{[1
]}

Wist, AD ^{[1
]}

Rabinowitz, P ^{[1
]}

Lehmann, KK ^{[1
]}

机构：

[1] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

来源：

APPLIED PHYSICS LETTERS | 2004年 / 85卷 / 19期

关键词：

D O I：

10.1063/1.1819520

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The implementation of cavity ring-down spectroscopy in an optical fiber resonator extends the viability of this highly sensitive technique for label-free detection of biological species. By chemically treating the surface of discrete tapered sensing regions along the length of a physically extended optical fiber resonator, we show single-cell sensitivity arising from optical scattering of the evanescent field surrounding the fiber. The observed detection limits, based on a minimum detectable scattering cross section on the order of 10 mum(2), suggest a broad range of new applications in a simple, inexpensive device for real-time cavity ring-down biosensing. (C) 2004 American Institute of Physics.

引用

页码：4523 / 4525

页数：3

共 21 条

[1] Quantitative spectroscopy analysis of prokaryotic cells:: vegetative cells and spores [J].