Self-assembling and auto-crosslinkable hyaluronic acid hydrogels with a fibrillar structure

被引：38

作者：

Palumbo, F. S. ^{[1
]}

Pitarresi, G. ^{[1
]}

Albanese, A. ^{[1
]}

Calascibetta, F. ^{[1
]}

Giammona, G. ^{[1
,2
]}

机构：

[1] Univ Palermo, Dipartimento Chim & Tecnol Farmaceut, I-90123 Palermo, Italy

[2] IBF CNR, I-90143 Palermo, Italy

来源：

ACTA BIOMATERIALIA | 2010年 / 6卷 / 01期

关键词：

Hyaluronic acid; L-Benzoyl-cysteine; Self-assembling; Oxidative crosslinking; Fibrillar structure; DRUG-DELIVERY; PEPTIDES; BIOMATERIALS; DERIVATIVES; GELATION; WATER;

D O I：

10.1016/j.actbio.2009.06.014

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A hyaluronic acid derivative bearing pendant L-benzoyl-cysteine portions (with a derivatization degree equal to 10 mol.%) was synthesized by linking N,N'-dibenzoyl-L-cystine to the polysaccharide and then reducing its disulfide bridge to thiol groups. The formation of pi-pi stacking interactions between the benzoyl moieties was studied by fluorescence spectroscopy as a function of polymer concentration and oxidation time. The efficiency of oxidation of thiol groups to disulfide bridges occurring in phosphate buffer pH 7.4, was determined by colorimetric assays. The hydrogel formed by means of oxidative crosslinking has shown the presence of fibrillar aggregates as detected by light and scanning electron microscopy. Human derm fibroblasts were encapsulated into hydrogel-forming solution, and their ability to proliferate was tested during 3 days of culture. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：195 / 204

页数：10

共 32 条

[1] A MODIFIED URONIC ACID CARBAZOLE REACTION [J].