Ratiometric sensing of Fe3+ through PET-CHEF-FRET processes: Live cell imaging, speciation and DFT studies

被引：27

作者：

Adhikari, Susanta ^{[1
]}

Ghosh, Avijit ^{[1
]}

Ghosh, Milan ^{[2
]}

Guria, Subhajit ^{[1
]}

Das, Debasis ^{[2
]}

机构：

[1] Univ Calcutta, Dept Chem, 92 APC Rd, Kolkata 700009, W Bengal, India

[2] Univ Burdwan, Dept Chem, Burdwan 713104, W Bengal, India

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 251卷

关键词：

Fluorescent sensor; Thymol; Proton transfer; DFT calculations; Cell imaging; Speciation studies; BOND ENERGY-TRANSFER; SELECTIVE FLUORESCENT SENSOR; IRON REGULATORY PROTEINS; TURN-ON; LIVING CELLS; RHODAMINE-B; TRANSFER CASSETTES; CHEMICAL SENSOR; HG2+ IONS; PROBE;

D O I：

10.1016/j.snb.2017.05.135

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

4-Hydroxy-5-isopropyl-2-methylisophthalaldehyde anchored rhodamine B derivative, RDDFT experiences Fe3+ assisted inhibition of photoinduced electron transfer (PET), chelation-enhanced fluorescence (CHEF) and fluorescence resonance energy transfer (FRET) process in aqueous buffered acetonitrile media. RDDFT is an excellent colorimetric Fe3+ probe that undergoes ratiometric fluorescence change to recognize and determine traces of Fe3+ in presence of Fe2+ in real sample, thus allowing speciation studies of iron. It detects as low as 2 x 10(-8) M Fe3+ with an association constant, 3.7025 x 106 M-1. Common ions do not show any significant interference in the detection of Fe3+ ion. RDDFT efficiently images intracellular Fe3+ in HeLa cells under fluorescence microscope. DFT studies strongly support experimental findings. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：942 / 950

页数：9

共 75 条

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