Ratiometric sensing of Fe3+ through PET-CHEF-FRET processes: Live cell imaging, speciation and DFT studies

被引：28
作者：
Adhikari, Susanta [1 ]
Ghosh, Avijit [1 ]
Ghosh, Milan [2 ]
Guria, Subhajit [1 ]
Das, Debasis [2 ]
机构：
[1] Univ Calcutta, Dept Chem, 92 APC Rd, Kolkata 700009, W Bengal, India
[2] Univ Burdwan, Dept Chem, Burdwan 713104, W Bengal, India
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 251卷
关键词：
Fluorescent sensor; Thymol; Proton transfer; DFT calculations; Cell imaging; Speciation studies; BOND ENERGY-TRANSFER; SELECTIVE FLUORESCENT SENSOR; IRON REGULATORY PROTEINS; TURN-ON; LIVING CELLS; RHODAMINE-B; TRANSFER CASSETTES; CHEMICAL SENSOR; HG2+ IONS; PROBE;
D O I：
10.1016/j.snb.2017.05.135
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
4-Hydroxy-5-isopropyl-2-methylisophthalaldehyde anchored rhodamine B derivative, RDDFT experiences Fe3+ assisted inhibition of photoinduced electron transfer (PET), chelation-enhanced fluorescence (CHEF) and fluorescence resonance energy transfer (FRET) process in aqueous buffered acetonitrile media. RDDFT is an excellent colorimetric Fe3+ probe that undergoes ratiometric fluorescence change to recognize and determine traces of Fe3+ in presence of Fe2+ in real sample, thus allowing speciation studies of iron. It detects as low as 2 x 10(-8) M Fe3+ with an association constant, 3.7025 x 106 M-1. Common ions do not show any significant interference in the detection of Fe3+ ion. RDDFT efficiently images intracellular Fe3+ in HeLa cells under fluorescence microscope. DFT studies strongly support experimental findings. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：942 / 950
页数：9
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