Analysis of noncovalent complexes between human telomeric DNA and polyamides containing N-methylpyrrole and N-methylimidazole by using electrospray ionization mass spectrometry

被引：12

作者：

Zhou, J ^{[1
]}

Yuan, G ^{[1
]}

机构：

[1] Peking Univ, Dept Biol Chem, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem, Beijing 100871, Peoples R China

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2005年 / 11卷 / 04期

关键词：

DNA recognition; human telomeric DNA; mass spectrometry; noncovalent interactions; polyamides;

D O I：

10.1002/chem.200400803

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Electrospray ionization mass spectrometry (ESI-MS) was used to investigate noncovalent complexes formed between four novel polyamides containing N-methylpyrrole (Py) and N-methylimidazole (1m), and human telomeric DNA. Of the four polyamides investigated, PyPyPygammaImImImbetaDp (3) had the highest binding affinity towards the duplex d(TTAGGGTTAGGG/CCCTAACCCTAA) (D1). Results of competition analysis showed that the polyamides had binding affinities with D1 in the order PyPyPygammaImImImbetaDp (3) > PyPyPyPygammaPyImImPybetaDp (4) > PyPyPybetaImImImbetaDp (2)>ImImImbetaDp (1). MS/MS spectra confirmed that binding between D1 and the hairpin polyamides is more stable than that with the three-ring polyamides. By contrast, in the case of single-stranded d(TTAGGGTTAGGG)(D2), the binding order changes to ImImImbetaDp (1)>PyPyPygammaImImImbetaDp (3) > PyPyPybetaImImImbetaDp (2).

引用

页码：1157 / 1162

页数：6

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