Frequency of azole resistance in clinical and environmental strains of Aspergillus fumigatus in Turkey: a multicentre study

被引：14

作者：

Ener, Beyza ^{[1
]}

Ergin, Cagri ^{[2
]}

Gulmez, Dolunay ^{[3
]}

Agca, Harun ^{[1
]}

Tikvesli, Melek ^{[4
]}

Aksoy, Secil Ak ^{[5
]}

Otkun, Muserref ^{[6
]}

Sig, Ali Korhan ^{[3
]}

Ogunc, Dilara ^{[7
]}

Ozhak, Betil ^{[7
]}

Topac, Tuncay ^{[8
]}

Ozdemir, Asli ^{[6
]}

Metin, Dilek Yesim ^{[9
]}

Polat, Suleyha Hilmioglu ^{[9
]}

Oz, Yasemin ^{[10
]}

Koc, Nedret ^{[11
]}

Atalay, Mustafa Altay ^{[11
]}

Erturan, Zayre ^{[12
]}

Birinci, Asuman ^{[13
]}

Cerikcioglu, Nilgun ^{[14
]}

Timur, Demet ^{[1
]}

Eksi, Fahriye ^{[15
]}

Genc, Gonca Erkose ^{[12
]}

Findik, Duygu ^{[16
]}

Gurcan, Saban ^{[4
]}

Kalkanci, Ayse ^{[17
]}

Arikan-Akdagli, Sevtap ^{[3
]}

机构：

[1] Bursa Uludag Univ, Fac Med, Dept Microbiol, Bursa, Turkey

[2] Pamukkale Univ, Fac Med, Dept Microbiol, Denizli, Turkey

[3] Hacettepe Univ, Fac Med, Dept Microbiol, Ankara, Turkey

[4] Trakya Univ, Fac Med, Dept Microbiol, Edirne, Turkey

[5] Bursa Uludag Univ, Inegol Vocat Sch, Bursa, Turkey

[6] Canakkale Onsekiz Mart Univ, Fac Med, Dept Microbiol, Canakkale, Turkey

[7] Akdeniz Univ, Fac Med, Dept Microbiol, Antalya, Turkey

[8] Sanliurfa Training & Res Hosp, Urfa, Turkey

[9] Ege Univ, Fac Med, Dept Microbiol, Izmir, Turkey

[10] Eskisehir Osmangazi Univ, Fac Med, Dept Microbiol, Eskisehir, Turkey

[11] Erciyes Univ, Fac Med, Dept Microbiol, Kayseri, Turkey

[12] Istanbul Univ, Istanbul Fac Med, Dept Microbiol, Istanbul, Turkey

[13] Ondokuz Mayis Univ, Fac Med, Dept Microbiol, Samsun, Turkey

[14] Marmara Univ, Fac Med, Dept Microbiol, Istanbul, Turkey

[15] Gaziantep Univ, Fac Med, Dept Microbiol, Gaziantep, Turkey

[16] Selcuk Univ, Fac Med, Dept Microbiol, Konya, Turkey

[17] Gazi Univ, Fac Med, Dept Microbiol, Ankara, Turkey

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2022年 / 77卷 / 07期

关键词：

D O I：

10.1093/jac/dkac125

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives Aspergillus fumigatus causes several diseases in humans and azole resistance in A. fumigatus strains is an important issue. The aim of this multicentre epidemiological study was to investigate the prevalence of azole resistance in clinical and environmental A. fumigatus isolates in Turkey. Methods Twenty-one centres participated in this study from 1 May 2018 to 1 October 2019. One participant from each centre was asked to collect environmental and clinical A. fumigatus isolates. Azole resistance was screened for using EUCAST agar screening methodology (EUCAST E.DEF 10.1) and was confirmed by the EUCAST E.DEF 9.3 reference microdilution method. Isolates with a phenotypic resistance pattern were sequenced for the cyp51A gene and microsatellite genotyping was used to determine the genetic relationships between the resistant strains. Results In total, resistance was found in 1.3% of the strains that were isolated from environmental samples and 3.3% of the strains that were isolated from clinical samples. Mutations in the cyp51A gene were detected in 9 (47.4%) of the 19 azole-resistant isolates, all of which were found to be TR34/L98H mutations. Microsatellite genotyping clearly differentiated the strains with the TR34/L98H mutation in the cyp51A gene from the strains with no mutation in this gene. Conclusions The rate of observed azole resistance of A. fumigatus isolates was low in this study, but the fact that more than half of the examined strains had the wild-type cyp51A gene supports the idea that other mechanisms of resistance are gradually increasing.

引用

页码：1894 / 1898

页数：5

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