Comparative physicochemical stability and efficacy study of lipoid S75-biopeptides nanoliposome composite produced by conventional and direct heating methods

被引:10
作者
Auwal, Shehu Muhammad [1 ,2 ]
Zarei, Mohammad [1 ,3 ]
Tand, Chin Ping [4 ]
Saari, Nazamid [1 ]
机构
[1] Univ Putra Malaysia, Fac Food Sci & Technol, Dept Food Sci, Serdang 43400, Selangor, Malaysia
[2] Bayero Univ, Fac Basic Med Sci, Dept Biochem, Kano, Nigeria
[3] Islamic Azad Univ, Coll Agr & Nat Resources, Dept Food Sci & Technol, Sanandaj Branch, Sanandaj, Iran
[4] Univ Putra Malaysia, Fac Food Sci & Technol, Dept Food Technol, Serdang, Selangor, Malaysia
关键词
ACE-inhibitory biopeptides; efficacy; nanoliposomes; stability; TEM; FTIR; INHIBITORY PEPTIDES; DRUG-DELIVERY; SOY LECITHIN; IN-VITRO; LIPOSOMES; ENCAPSULATION; RELEASE; BIOAVAILABILITY; MEMBRANES; FRACTION;
D O I
10.1080/10942912.2018.1504064
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Liposomes are used as effective nanodelivery devices to improve the physicochemical stability and biological efficacy of the encapsulated peptides and proteins. In this study, nanoliposome composite of lipoid S75-entrapped angiotensin 1-converting enzyme (ACE)-inhibitory biopeptides was prepared by conventional (BLS75-CM) and direct heating (BLS75-DHM) methods. The nanoliposomes (BLS75-CM and BLS75-DHM) were stored at 4 degrees C for 8 weeks and evaluated for physicochemical stability in terms of particle size, polydispersity index (pdi), zeta potential, and encapsulation efficiency (EE). These were also studied for residual ACE-inhibitory efficacy following their digestion under simulated gastrointestinal tract condition. The BLS75-CM was found to maintain higher physicochemical stability in terms of particle size, pdi, and zeta potential compared to BLS75-DHM. However, the BLS75-DHM indicated higher EE and efficacy with greater residual ACE-inhibitory activity of 47.37% compared to 44.18% and 36.84% that were obtained for the digested BLS75-CM and digested biopeptides without encapsulation, respectively. In vitro release study showed a cumulative biopeptides release of 66.41% and 69.00% from BLS75-CM and BLS75-DHM, respectively. The results of transmission electron microscopy showed spherical appearance of the nanoliposome capsules while Fourier Transform Infrared spectroscopy indicated the presence of ionic complexation and hydrogen bonds between the biopeptides and their phospholipid matrix.
引用
收藏
页码:1646 / 1660
页数:15
相关论文
共 55 条
[1]   Improved Stability and Antidiabetic Potential of Insulin Containing Folic Acid Functionalized Polymer Stabilized Multi layered Liposomes Following Oral Administration [J].
Agrawal, Ashish Kumar ;
Harde, Harshad ;
Thanki, Kaushik ;
Jain, Sanyog .
BIOMACROMOLECULES, 2014, 15 (01) :350-360
[2]   Challenges and Solutions to Incorporation of Nutraceuticals in Foods [J].
Augustin, Mary Ann ;
Sanguansri, Luz .
ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 6, 2015, 6 :463-477
[3]   Improved In Vivo Efficacy of Anti-Hypertensive Biopeptides Encapsulated in Chitosan Nanoparticles Fabricated by Ionotropic Gelation on Spontaneously Hypertensive Rats [J].
Auwal, Shehu Muhammad ;
Zarei, Mohammad ;
Tan, Chin Ping ;
Basri, Mahiran ;
Saari, Nazamid .
NANOMATERIALS, 2017, 7 (12)
[4]   Response Surface Optimisation for the Production of Antioxidant Hydrolysates from Stone Fish Protein Using Bromelain [J].
Auwal, Shehu Muhammad ;
Zarei, Mohammad ;
Abdul-Hamid, Azizah ;
Saari, Nazamid .
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2017, 2017
[5]   Optimization of Bromelain-Aided Production of Angiotensin I-Converting Enzyme Inhibitory Hydrolysates from Stone Fish Using Response Surface Methodology [J].
Auwal, Shehu Muhammad ;
Zarei, Mohammad ;
Abdul-Hamid, Azizah ;
Saari, Nazamid .
MARINE DRUGS, 2017, 15 (04)
[6]   Liposomes as nanomedical devices [J].
Bozzuto, Giuseppina ;
Molinari, Agnese .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2015, 10 :975-999
[7]   Preparation and characterisation of nanoliposomes containing winged bean seeds bioactive peptides [J].
Chay, Shyan Yea ;
Tan, Wei Kiat ;
Saari, Nazamid .
JOURNAL OF MICROENCAPSULATION, 2015, 32 (05) :488-495
[8]   The Stability, Sustained Release and Cellular Antioxidant Activity of Curcumin Nanoliposomes [J].
Chen, Xing ;
Zou, Li-Qiang ;
Niu, Jing ;
Liu, Wei ;
Peng, Sheng-Feng ;
Liu, Cheng-Mei .
MOLECULES, 2015, 20 (08) :14293-14311
[9]   Enzymatic protein hydrolysates in human nutrition [J].
Clemente, A .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2000, 11 (07) :254-262
[10]   Direct comparison of two pegylated liposomal doxorubicin formulations: Is AUC predictive for toxicity and efficacy? [J].
Cui, JingXia ;
Li, ChunLei ;
Guo, WenMin ;
Li, YanHui ;
Wang, CaiXia ;
Zhang, Li ;
Zhang, Lan ;
Hao, Yanli ;
Wang, YongLi .
JOURNAL OF CONTROLLED RELEASE, 2007, 118 (02) :204-215