Bombesin functionalized 64Cu-copper sulfide nanoparticles for targeted imaging of orthotopic prostate cancer

被引：27
作者：
Cai, Huawei [1 ,2 ]
Xie, Fang [2 ,3 ,4 ,5 ]
Mulgaonkar, Aditi [2 ]
Chen, Lihong [6 ]
Sun, Xiankai [2 ,4 ]
Hsieh, Jer-Tsong [7 ]
Peng, Fangyu [2 ,3 ,4 ]
Tian, Rong [1 ]
li, Lin [1 ]
Wu, Changqiang [8 ,9 ,10 ]
Ai, Hua [10 ]
机构：
[1] Sichuan Univ, West China Hosp, Dept Nucl Med, Lab Clin Nucl Med, Chengdu 610041, Sichuan, Peoples R China
[2] Univ Texas Southwestern Med Ctr Dallas, Dept Radiol, Dallas, TX 75390 USA
[3] Univ Texas Southwestern Med Ctr Dallas, Harold C Simmons Comprehens Canc Ctr, Dallas, TX 75390 USA
[4] Univ Texas Southwestern Med Ctr Dallas, Adv Imaging Res Ctr, Dallas, TX 75390 USA
[5] Fudan Univ, Huashan Hosp, PET Ctr, Shanghai 200040, Peoples R China
[6] Sichuan Univ, West China Sch Basic Sci & Forens Med, Dept Biochem & Mol Biol, Chengdu 610041, Sichuan, Peoples R China
[7] Univ Texas Southwestern Med Ctr Dallas, Dept Urol, Dallas, TX 75390 USA
[8] North Sichuan Med Coll, Sichuan Key Lab Med Imaging, Nanchong 637000, Peoples R China
[9] North Sichuan Med Coll, Sch Med Imaging, Nanchong 637000, Peoples R China
[10] Sichuan Univ, Natl Engn Res Ctr Biomat, Chengdu 610064, Sichuan, Peoples R China
来源：
NANOMEDICINE | 2018年 / 13卷 / 14期
关键词：
bombesin; Cu-64-copper sulfide nanoparticles; micro-PET-CT; prostate cancer; POSITRON-EMISSION-TOMOGRAPHY; GOLD NANOPARTICLES; EAU GUIDELINES; IN-VITRO; MICE; ANALOGS; MODEL;
D O I：
10.2217/nnm-2018-0062
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aim: To synthesize and evaluate the imaging potential of Bom-PEG-[Cu-64] CuS nanoparticles (NPs) in orothotopic prostate tumor. Materials & methods: [Cu-64] CuS NPs were synthesized in aqueous solution by (CuCl2)-Cu-64 and Na2S reaction. Then PEG linker with or without bombesin peptide were conjugated to the surface of [Cu-64] CuS NPs to produce Bom-PEG-[Cu-64] CuS and PEG-[Cu-64] CuS NPs. These two kinds of NPs were used for testing specific uptake in prostate cancer cells in vitro and imaging of orthotopic prostate tumor in vivo. Results: Bom-PEG-[Cu-64] CuS and PEG-[Cu-64] CuS NPs were successfully synthesized with core diameter of approximately 5 nm. Radioactive cellular uptake revealed that Bom-PEG-[Cu-64] CuS was able to specifically bind to prostate cancer cells, and the microPET-CT imaging indicated clear visualization of orthotopic prostate tumors. Conclusion: Radiolabeled Bom-PEG-[Cu-64] CuS NPs have potential as an ideal agent for orthotopic prostate tumor imaging by microPET-CT.
引用
收藏
页码：1695 / 1705
页数：11
相关论文
共 25 条
  • [11] Ma MT, 2011, CURR TOP MED CHEM, V11, P500
  • [12] Bombesin analogues for gastrin-releasing peptide receptor imaging
    Nanda, Prasant K.
    Pandey, Usha
    Bottenus, Brienne N.
    Rold, Tammy L.
    Sieckman, Gary L.
    Szczodroski, Ashley F.
    Hoffman, Timothy J.
    Smith, Charles J.
    [J]. NUCLEAR MEDICINE AND BIOLOGY, 2012, 39 (04) : 461 - 471
  • [13] An Orthotopic Murine Model of Human Prostate Cancer Metastasis
    Pavese, Janet
    Ogden, Irene M.
    Bergan, Raymond C.
    [J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2013, (79): : e50873
  • [14] Gastrin-releasing peptide receptor-based targeting using bombesin analogues is superior to metabolism-based targeting using choline for in vivo imaging of human prostate cancer xenografts
    Schroeder, Rogier P. J.
    van Weerden, W. M.
    Krenning, E. P.
    Bangma, C. H.
    Berndsen, S.
    Grievink-de Ligt, C. H.
    Groen, H. C.
    Reneman, S.
    de Blois, E.
    Breeman, W. A. P.
    de Jong, M.
    [J]. EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2011, 38 (07) : 1257 - 1266
  • [15] Cancer Statistics, 2014
    Siegel, Rebecca
    Ma, Jiemin
    Zou, Zhaohui
    Jemal, Ahmedin
    [J]. CA-A CANCER JOURNAL FOR CLINICIANS, 2014, 64 (01) : 9 - 29
  • [16] Current clinical challenges in prostate cancer
    Silberstein, Jonathan L.
    Pal, Sumanta Kumar
    Lewis, Brian
    Sartor, Oliver
    [J]. TRANSLATIONAL ANDROLOGY AND UROLOGY, 2013, 2 (03) : 122 - 136
  • [17] Intravital Imaging of Human Prostate Cancer Using Viral Nanoparticles Targeted to Gastrin-Releasing Peptide Receptors
    Steinmetz, Nicole F.
    Ablack, Amber L.
    Hickey, Jennifer L.
    Ablack, Jailal
    Manocha, Bhavik
    Mymryk, Joe S.
    Luyt, Leonard G.
    Lewis, John D.
    [J]. SMALL, 2011, 7 (12) : 1664 - 1672
  • [18] An orthotopic metastatic prostate cancer model in SCID mice via grafting of a transplantable human prostate tumor line
    Wang, YW
    Xue, H
    Cutz, JC
    Bayani, J
    Mawji, NR
    Chen, WG
    Goetz, LJ
    Hayward, SW
    Sadar, MD
    Gilks, CB
    Gout, PW
    Squire, JA
    Cunha, GR
    Wang, YZ
    [J]. LABORATORY INVESTIGATION, 2005, 85 (11) : 1392 - 1404
  • [19] CuS nanoparticles: clinically favorable materials for photothermal applications?
    Xiao, Zeyu
    [J]. NANOMEDICINE, 2014, 9 (03) : 373 - 375
  • [20] Role of DAB2IP in modulating epithelial-to-mesenchymal transition and prostate cancer metastasis
    Xie, Daxing
    Gore, Crystal
    Liu, Jun
    Pong, Rey-Chen
    Mason, Ralph
    Hao, Guiyang
    Long, Michael
    Kabbani, Wareef
    Yu, Luyang
    Zhang, Haifeng
    Chen, Hong
    Sun, Xiankai
    Boothman, David A.
    Min, Wang
    Hsieh, Jer-Tsong
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (06) : 2485 - 2490
123