Bombesin functionalized 64Cu-copper sulfide nanoparticles for targeted imaging of orthotopic prostate cancer

被引：27
作者：
Cai, Huawei [1 ,2 ]
Xie, Fang [2 ,3 ,4 ,5 ]
Mulgaonkar, Aditi [2 ]
Chen, Lihong [6 ]
Sun, Xiankai [2 ,4 ]
Hsieh, Jer-Tsong [7 ]
Peng, Fangyu [2 ,3 ,4 ]
Tian, Rong [1 ]
li, Lin [1 ]
Wu, Changqiang [8 ,9 ,10 ]
Ai, Hua [10 ]
机构：
[1] Sichuan Univ, West China Hosp, Dept Nucl Med, Lab Clin Nucl Med, Chengdu 610041, Sichuan, Peoples R China
[2] Univ Texas Southwestern Med Ctr Dallas, Dept Radiol, Dallas, TX 75390 USA
[3] Univ Texas Southwestern Med Ctr Dallas, Harold C Simmons Comprehens Canc Ctr, Dallas, TX 75390 USA
[4] Univ Texas Southwestern Med Ctr Dallas, Adv Imaging Res Ctr, Dallas, TX 75390 USA
[5] Fudan Univ, Huashan Hosp, PET Ctr, Shanghai 200040, Peoples R China
[6] Sichuan Univ, West China Sch Basic Sci & Forens Med, Dept Biochem & Mol Biol, Chengdu 610041, Sichuan, Peoples R China
[7] Univ Texas Southwestern Med Ctr Dallas, Dept Urol, Dallas, TX 75390 USA
[8] North Sichuan Med Coll, Sichuan Key Lab Med Imaging, Nanchong 637000, Peoples R China
[9] North Sichuan Med Coll, Sch Med Imaging, Nanchong 637000, Peoples R China
[10] Sichuan Univ, Natl Engn Res Ctr Biomat, Chengdu 610064, Sichuan, Peoples R China
来源：
NANOMEDICINE | 2018年 / 13卷 / 14期
关键词：
bombesin; Cu-64-copper sulfide nanoparticles; micro-PET-CT; prostate cancer; POSITRON-EMISSION-TOMOGRAPHY; GOLD NANOPARTICLES; EAU GUIDELINES; IN-VITRO; MICE; ANALOGS; MODEL;
D O I：
10.2217/nnm-2018-0062
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aim: To synthesize and evaluate the imaging potential of Bom-PEG-[Cu-64] CuS nanoparticles (NPs) in orothotopic prostate tumor. Materials & methods: [Cu-64] CuS NPs were synthesized in aqueous solution by (CuCl2)-Cu-64 and Na2S reaction. Then PEG linker with or without bombesin peptide were conjugated to the surface of [Cu-64] CuS NPs to produce Bom-PEG-[Cu-64] CuS and PEG-[Cu-64] CuS NPs. These two kinds of NPs were used for testing specific uptake in prostate cancer cells in vitro and imaging of orthotopic prostate tumor in vivo. Results: Bom-PEG-[Cu-64] CuS and PEG-[Cu-64] CuS NPs were successfully synthesized with core diameter of approximately 5 nm. Radioactive cellular uptake revealed that Bom-PEG-[Cu-64] CuS was able to specifically bind to prostate cancer cells, and the microPET-CT imaging indicated clear visualization of orthotopic prostate tumors. Conclusion: Radiolabeled Bom-PEG-[Cu-64] CuS NPs have potential as an ideal agent for orthotopic prostate tumor imaging by microPET-CT.
引用
收藏
页码：1695 / 1705
页数：11
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