Structure and gene cluster of the O-antigen of Enterobacter cloacae G3421

被引：7

作者：

Perepelov, Andrei V. ^{[1
]}

Filatov, Andrei V. ^{[1
]}

Wang, Min ^{[2
,3
]}

Shashkov, Alexander S. ^{[1
]}

Wang, Lei ^{[2
,3
]}

Knirel, Yuriy A. ^{[1
]}

机构：

[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia

[2] Nankai Univ, TEDA, Inst Biol Sci & Biotechnol, Tianjin 300457, Peoples R China

[3] Nankai Univ, Coll Life Sci, Minist Educ, Key Lab Mol Microbiol & Technol, Tianjin 300071, Peoples R China

来源：

CARBOHYDRATE RESEARCH | 2016年 / 427卷

基金：

俄罗斯基础研究基金会; 中国国家自然科学基金;

关键词：

Enterobacter cloacae; Bacterial polysaccharide structure; Solvolytic cleavage; Lipopolysaccharide; O-antigen gene cluster; SHIGELLA-BOYDII; POLYSACCHARIDES; OVEREXPRESSION;

D O I：

10.1016/j.carres.2016.03.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G3421 and studied by sugar analysis along with 1D and 2D H-1 and C-13 NMR spectroscopy. In addition, partial solvolysis with anhydrous trifluoroacetic acid was applied, which cleaved selectively the alpha-L-rhamnopyranosidic linkages. The following structure of the branched hexasaccharide repeating unit was established. [GRAPHICS] The O-polysaccharide studied shares the beta-l-Rhap-(1 -> 4)-alpha-l-Rhap-(1 -> 2)-alpha-l-Rhap trisaccharide fragment with the O-polysaccharide of Shigella boydii type 18. The O-antigen gene cluster of E. cloacae G3421 was sequenced. Functions of genes in the cluster, including those for glycosyltransferases, were tentatively assigned by a comparison with sequences in the available databases and found to be consistent with the O-polysaccharide structure. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：55 / 59

页数：5

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