Mechanism for the peroxynitrite scavenging activity by anthocyanins

被引：81

作者：

Tsuda, T

Kato, Y

Osawa, T

机构：

[1] Tokaigakuen Womens Coll, Tenpaku Ku, Nagoya, Aichi 4688514, Japan

[2] Himeji Inst Technol, Sch Humanities Environm Policy & Technol, Himeji, Hyogo 6700092, Japan

[3] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan

来源：

FEBS LETTERS | 2000年 / 484卷 / 03期

关键词：

anthocyanin; nitric oxide; peroxynitrite; 3-nitrotyrosine; antioxidant;

D O I：

10.1016/S0014-5793(00)02150-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that anthocyanins can function as potent inhibitors of the formation of nitrated tyrosine in vitro, and clarified how pelargonidin (Pel), which has a mono-hydroxyl group on the B-ring, can scavenge peroxynitrite (ONOO-) by detection of the reaction products, Pel was reacted with ONOO-, then the reaction mixture was analyzed using highperformance liquid chromatography (HPLC). The HPLC analyses showed two novel peaks assumed to be the reaction products. Based on the instrumental analyses, the reaction products were identified as p-hydroxybenzoic acid and 4-hydroxy-3-nitrobenzoic acid. Pel can protect tyrosine from undergoing nitration through the formation of p-hydroxybenzoic acid and 4-hydroxy-3-nitrobenzoic acid. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B,V, All rights reserved.

引用

页码：207 / 210

页数：4

共 27 条

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