Expression of microRNA-155 precursor in peripheral blood mononuclear cells from hepatitis C patients after antiviral treatment

被引：21

作者：

Sidorkiewicz, M. ^{[1
]}

Grek, M. ^{[1
]}

Jozwiak, B. ^{[1
]}

Majda-Stanislawska, E. ^{[2
]}

Piekarska, A. ^{[2
]}

Bartkowiak, J. ^{[1
]}

机构：

[1] Med Univ Lodz, Dept Med Biochem, PL-90215 Lodz, Poland

[2] Med Univ Lodz, Dept Infect Dis & Hepatol, PL-90215 Lodz, Poland

来源：

ACTA VIROLOGICA | 2010年 / 54卷 / 01期

关键词：

Hepatitis C virus; microRNA-155; peripheral blood mononuclear cells; BIC; antiviral treatment; VIRUS HCV; RNA; PERSISTENCE; INTERFERON; MODULATION; RIBAVIRIN; SUBTYPES; HODGKIN; MIR-155;

D O I：

10.4149/av_2010_01_75

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Chronic hepatitis caused by Hepatitis C virus (HCV) is the main source of liver cirrhosis, hepatocellular carcinoma, and extra-hepatic diseases. After treatment-induced resolution of hepatitis C, the persistence of HCV RNA in serum and peripheral blood mononuclear cells (PBMCs) is often observed. An expression of the precursor of microRNA-155 (miR-155) called BIC can be the factor responsible for a course of HCV infection. Therefore, we assessed the relationship between BIC expression and HCV RNA status in sera and PBMCs samples of 64 hepatitis C patients treated with interferon alpha (IFN-alpha) + ribavirin. High expression of BIC in PBMCs was determined in 100% of patients that harbored HCV RNA in serum and PBMCs. Further, we found that 83% of PBMCs samples were BIC-positive in a group of patients that eliminated HCV RNA only from serum. The lowest expression of BIC was found in patients that eliminated HCV RNA from both serum and PBMCs.

引用

页码：75 / 78

页数：4

共 24 条

[1] Continuous release of hepatitis C virus (HCV) by peripheral blood mononuclear cells and B-lymphoblastoid cell-line cultures derived from HCV-infected patients [J].

Baré, P ;

Massud, I ;

Parodi, C ;

Belmonte, L ;

García, G ;

Nebel, MC ;

Corti, M ;

Pinto, MT ;

Bianco, RP ;

Bracco, MM ;

Campos, R ;

Ares, BR .

JOURNAL OF GENERAL VIROLOGY, 2005, 86 :1717-1727

[2] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[3] Extrahepatic replication of HCV: Insights into clinical manifestations and biological consequences [J].

Blackard, Jason T. ;

Kemmer, Nyingi ;

Sherman, Kenneth E. .

HEPATOLOGY, 2006, 44 (01) :15-22

[4]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[5] Hepatitis C virus (HCV) infection:: A systemic disease [J].

Craxi, Antonio ;

Laffi, Giacomo ;

Zignego, Anna Linda .

MOLECULAR ASPECTS OF MEDICINE, 2008, 29 (1-2) :85-95

[6] Identification, using cDNA macroarray analysis, of distinct gene expression profiles associated with pathological and virological features of hepatocellular carcinoma [J].

Delpuech, O ;

Trabut, JB ;

Carnot, F ;

Feuillard, J ;

Brechot, C ;

Kremsdorf, D .

ONCOGENE, 2002, 21 (18) :2926-2937

[7]

DULAK J, 1996, HEPATOL POL, V3, P31

[8] Accumulation of miR-155 and BIC RNA in human B cell lymphomas [J].

Eis, PS ;

Tam, W ;

Sun, LP ;

Chadburn, A ;

Li, ZD ;

Gomez, MF ;

Lund, E ;

Dahlberg, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (10) :3627-3632

[9] Persistence of hepatitis C virus in peripheral blood mononuclear cells of sustained viral responders to pegylated interferon and ribavirin therapy [J].

Gallegos-Orozco, Juan F. ;

Rakela, Jorge ;

Rosati, Marianne J. ;

Vargas, Hugo E. ;

Balan, Vijayan .

DIGESTIVE DISEASES AND SCIENCES, 2008, 53 (09) :2564-2568

[10] T cell activation induces a noncoding RNA transcript sensitive to inhibition by immunosuppressant drugs and encoded by the proto-oncogene, BIC [J].

Haasch, D ;

Chen, YW ;

Reilly, RM ;

Chiou, XG ;

Koterski, S ;

Smith, ML ;

Kroeger, P ;

McWeeny, K ;

Halbert, DN ;

Mollison, KW ;

Djuric, SW ;

Trevillyan, JM .

CELLULAR IMMUNOLOGY, 2002, 217 (1-2) :78-86

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