Isolation and characterization of microsatellite markers in Japanese pear (Pyrus pyrifolia Nakai)

被引:30
作者
Inoue, Eiichi
Matsuki, Yumi
Anzai, Hiroyuki
Evans, Kate
机构
[1] Ibaraki Univ, Coll Agr, Ami, Ibaraki 3000393, Japan
[2] Ibaraki Univ, Ctr Gene Res, Ami, Ibaraki 3000393, Japan
[3] E Malling Res, Maidstone ME19 6BJ, Kent, England
来源
MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 03期
关键词
microsatellites; pear; Pyrus; Rosaceae;
D O I
10.1111/j.1471-8286.2006.01612.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fourteen microsatellite markers were developed from an enriched genomic library of Japanese pear (Pyrus pyrifolia) by selective hybridization. They were characterized using 17 Japanese pear cultivars. The expected heterozygosity and observed heterozygosity ranged from 0.21 to 0.74 and from 0 to 0.88, respectively. Two to 11 alleles were detected per locus, with IPPN09 and IPPN15 judged to amplify multiple loci. IPPN17 was the most informative locus with the lowest probability of identity (0.19). These primers exhibited a high cross-species transferability between species and genera.
引用
收藏
页码:445 / 447
页数:3
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