Antibacterial and Antibiofilm Activity and Mode of Action of Magainin 2 against Drug-Resistant Acinetobacter baumannii

被引：84

作者：

Kim, Min Kyung ^{[1
]}

Kang, Na Hee ^{[1
]}

Ko, Su Jin ^{[1
]}

Park, Jonggwan ^{[2
]}

Park, Eunji ^{[1
]}

Shin, Dong Won ^{[3
]}

Kim, Seo Hyun ^{[3
]}

Lee, Seung A. ^{[3
]}

Lee, Ji In ^{[3
]}

Lee, Seung Hyun ^{[3
]}

Ha, Eun Gi ^{[3
]}

Jeon, Seung Hun ^{[3
]}

Park, Yoonkyung ^{[1
,4
]}

机构：

[1] Chosun Univ, Dept Biomed Sci, Gwangju 61452, South Korea

[2] Kongju Natl Univ, Dept Bioinformat, Kong Ju 38065, South Korea

[3] Jangseong High Sch, Jeollanamdo 57216, South Korea

[4] Chosun Univ, Res Ctr Proteineous Mat, Gwangju 61452, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2018年 / 19卷 / 10期

基金：

新加坡国家研究基金会;

关键词：

Acinetobacter baumannii; multidrug-resistant; antimicrobial peptide; antibiofilm activity; physiological salt; ANTIMICROBIAL PEPTIDES; INFECTIOUS-DISEASES; BIOFILM FORMATION; ESKAPE PATHOGENS; PORE FORMATION; NO ESKAPE; SKIN; EPIDEMIOLOGY; INHIBITORS; MECHANISM;

D O I：

10.3390/ijms19103041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antimicrobial peptides (AMPs) are promising therapeutic agents for treating antibiotic-resistant bacterial infections. Previous studies showed that magainin 2 (isolated from African clawed fogs Xenopus laevis) has antimicrobial activity against gram-positive and gram-negative bacteria. The present study was conducted to investigate the antibacterial activity of magainin 2 against Acinetobacter baumannii. Magainin 2 showed excellent antibacterial activity against A. baumannii strains and high stability at physiological salt concentrations. This peptide was not cytotoxic towards HaCaT cells and showed no hemolytic activity. Biofilm inhibition and elimination were significantly induced in all A. baumannii strains exposed to magainin 2. We confirmed the mechanism of magainin 2 on the bacterial outer and inner membranes. Collectively, these results suggest that magainin 2 is an effective antimicrobial and antibiofilm agent against A. baumannii strains.

引用

页数：14

共 53 条

[1] Biofilm formation in clinical isolates of nosocomial Acinetobacter baumannii and its relationship with multidrug resistance [J].

Babapour, Ebrahim ;

Haddadi, Azam ;

Mirnejad, Reza ;

Angaji, Seyed-Abdolhamid ;

Amirmozafari, Nour .

ASIAN PACIFIC JOURNAL OF TROPICAL BIOMEDICINE, 2016, 6 (06) :528-533

[2]

BAKER MA, 1993, CANCER RES, V53, P3052

[3] Bad Bugs, No Drugs: No ESKAPE! An Update from the Infectious Diseases Society of America [J].

Boucher, Helen W. ;

Talbot, George H. ;

Bradley, John S. ;

Edwards, John E., Jr. ;

Gilbert, David ;

Rice, Louis B. ;

Scheld, Michael ;

Spellberg, Brad ;

Bartlett, John .

CLINICAL INFECTIOUS DISEASES, 2009, 48 (01) :1-12

[4] Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? [J].

Brogden, KA .

NATURE REVIEWS MICROBIOLOGY, 2005, 3 (03) :238-250

[5]

Cho Eugene, 2018, Oncotarget, V9, P7902, DOI 10.18632/oncotarget.24083

[6] Nosocomial bacteremia due to Acinetobacter baumannii:: epidemiology, clinical features and treatment [J].

Cisneros, JM ;

Rodríguez-Baño, J .

CLINICAL MICROBIOLOGY AND INFECTION, 2002, 8 (11) :687-693

[7] Antibiotics and Bacterial Resistance in the 21st Century [J].

Fair, Richard J. ;

Tor, Yitzhak .

PERSPECTIVES IN MEDICINAL CHEMISTRY, 2014, 6 :25-64

[8] Infectious diseases: Considerations for the 21st century [J].

Fauci, AS .

CLINICAL INFECTIOUS DISEASES, 2001, 32 (05) :675-685

[9] Fighting bacterial infections-Future treatment options [J].

Fernebro, Jenny .

DRUG RESISTANCE UPDATES, 2011, 14 (02) :125-139

[10] SOLID-PHASE PEPTIDE-SYNTHESIS UTILIZING 9-FLUORENYLMETHOXYCARBONYL AMINO-ACIDS [J].

FIELDS, GB ;

NOBLE, RL .

INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 1990, 35 (03) :161-214

← 1 2 3 4 5 6 →