Polychlorinated biphenyl detection in organic solvents with paper-based analytical devices

Sample pretreatment process is usually performed before real sample detection. Extraction is one of the most prevalent pretreatment methods. Due to the unequal solubility in two liquid phases, target is extracted (usually from water to organic phase) and further detected with laboratory instruments. Although the analytical instruments have high sensitivity and selectivity, they are relatively expensive and require skilful hands, which is restricted to laboratory use. Here, paper-based analytical devices (mu PADs) for measuring samples extracted with organic solvents were tested. The mu PADs were employed as a filter for buffer exchange, allowing the transfer of targets from organic solvent to water-based buffer, which could be specifically detected by water-based assays. As an example, polychlorinated biphenyls (PCBs) in n-hexane were detected with aptamer and gold nanoparticles (AuNPs) on mu PADs within 30 min. The result of colour change can be recognized from 70.0 ng/L with a low loading amount (0.5 mu L). Instead of laboratory instrumental analysis with relative high cost, this assay showed the promising utility of mu PADs for target detection after sample pretreatment, which could be utilized as a general platform for inexpensive onsite detection.