Protein crystal growth in the advanced protein crystallization facility on the LMS mission: a comparison of Sulfolobus solfataricus alcohol dehydrogenase crystals grown on the ground and in microgravity

被引：24

作者：

Esposito, L

Sica, F

Sorrentino, G

Berisio, R

Carotenuto, L

Giordano, A

Raia, CA

Rossi, M

Lamzin, VS

Wilson, KS

Zagari, A

机构：

[1] Univ Naples, CNR, Ctr Studio Biocristallog, I-80134 Naples, Italy

[2] Univ Naples, Dipartimento Chim, I-80134 Naples, Italy

[3] Micrograv Adv Res & Support Ctr, I-80144 Naples, Italy

[4] CNR, Ist Biochim Prot & Enzimol, Naples, Italy

[5] DESY, European Mol Biol Lab, D-22603 Hamburg, Germany

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1998年 / 54卷

关键词：

D O I：

10.1107/S0907444997011992

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of alcohol dehydrogenase from Sulfolobus solfataricus were grown in the Advanced Protein Crystallization Facility during the Life and Microgravity Sciences Spacelab mission on the US Space Shuttle. Large diffracting crystals were obtained by dialysis, whereas only poor-quality crystals were obtained by vapour diffusion. The quality of both the microgravity and ground-based crystals was analysed by X-ray diffraction. There was some improvement in terms of size and diffraction resolution limit for the microgravity crystals. However, the twinning observed in the Earth-grown crystals was also present for those grown in microgravity.

引用

页码：386 / 390

页数：5

共 20 条

[1] EXPERIMENT EQUIPMENT FOR PROTEIN CRYSTALLIZATION IN MU-G FACILITIES [J].