MoSnt2-dependent deacetylation of histone H3 mediates MoTor-dependent autophagy and plant infection by the rice blast fungus Magnaporthe oryzae

被引:95
作者
He, Min [1 ,2 ,3 ]
Xu, Youpin [1 ,2 ]
Chen, Jinhua [1 ,2 ]
Luo, Yuan [1 ,2 ]
Lv, Yang [1 ,2 ]
Su, Jia [1 ,2 ]
Kershaw, Michael J. [3 ]
Li, Weitao [1 ,2 ]
Wang, Jing [1 ,2 ]
Yin, Junjie [1 ,2 ]
Zhu, Xiaobo [1 ,2 ]
Liu, Xiaohong [4 ]
Chern, Mawsheng [5 ]
Ma, Bingtian [1 ,2 ]
Wang, Jichun [1 ,2 ]
Qin, Peng [1 ,2 ]
Chen, Weilan [1 ,2 ]
Wang, Yuping [1 ,2 ]
Wang, Wenming [1 ,2 ]
Ren, Zhenglong [1 ,2 ]
Wu, Xianjun [1 ,2 ]
Li, Ping [1 ,2 ]
Li, Shigui [1 ,2 ]
Peng, Youliang [6 ]
Lin, Fucheng [4 ]
Talbot, Nicholas J. [3 ]
Chen, Xuewei [1 ,2 ]
机构
[1] Sichuan Agr Univ, Rice Res Inst, State Key Lab Hybrid Rice, Key Lab Major Crop Dis, Chengdu, Sichuan, Peoples R China
[2] Sichuan Agr Univ, Rice Res Inst, Collaborat Innovat Ctr Hybrid Rice Yangtze River, Chengdu, Sichuan, Peoples R China
[3] Univ Exeter, Sch Biosci, Exeter, Devon, England
[4] Zhejiang Univ, Biotechnol Inst, State Key Lab Rice Biol, Hangzhou, Zhejiang, Peoples R China
[5] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
[6] China Agr Univ, State Key Lab Agrobiotechnol & MOA, Key Lab Plant Pathol, Beijing, Peoples R China
基金
英国生物技术与生命科学研究理事会; 中国国家自然科学基金;
关键词
Autophagy; Magnaporthe oryzae; MoSnt2; MoTor signaling; pathogenicity; TRANSCRIPTION FACTOR; ASEXUAL DIFFERENTIATION; EPIGENETIC REGULATION; PATHOGENICITY; ACETYLATION; COMPLEX; YEAST; SNT2; INACTIVATION; PATHOGENESIS;
D O I
10.1080/15548627.2018.1458171
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Autophagy is essential for appressorium-mediated plant infection by Magnaporthe oryzae, the causal agent of rice blast disease and a major threat to global food security. The regulatory mechanism of pathogenicity-associated autophagy, however, remains largely unknown. Here, we report the identification and functional characterization of a plausible ortholog of yeast SNT2 in M. oryzae, which we term MoSNT2. Deletion mutants of MoSNT2 are compromised in autophagy homeostasis and display severe defects in autophagy-dependent fungal cell death and pathogenicity. These mutants are also impaired in infection structure development, conidiation, oxidative stress tolerance and cell wall integrity. MoSnt2 recognizes histone H3 acetylation through its PHD1 domain and thereby recruits the histone deacetylase complex, resulting in deacetylation of H3. MoSnt2 binds to promoters of autophagy genes MoATG6, 15, 16, and 22 to regulate their expression. In addition, MoTor controls MoSNT2 expression to regulate MoTor signaling which leads to autophagy and rice infection. Our study provides evidence of a direct link between MoSnt2 and MoTor signaling and defines a novel epigenetic mechanism by which MoSNT2 regulates infection-associated autophagy and plant infection by the rice blast fungus.
引用
收藏
页码:1543 / 1561
页数:19
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