Galantamine blocks cloned Kv2.1, but not Kv 1.5 potassium channels

Galantamine is a cholinesterase inhibitor (AChEI) currently used in treatment of Alzheimer's disease (AD). In the present study, the effects of galantamine on currents of cloned Kv2.1 and Kv1.5 potassium channels were investigated by using patch-clamp whole cell recording techniques. Kv2.1 and Kvl.5 were stably expressed in HEK293 cells. Galantamine blocked Kv2.1 current in a concentration-dependent manner. When depolarizing from -50 to +40 mV, the IC50 of galantamine for inhibition of Kv2.1 was 5.6 muM. Galantamine 10 muM shifted the activation curve of Kv2.1 to negative potential by 4.0 mV At the same concentration, galantamine shifted the inactivation curve to negative potential by 25.2 mV. While Kv1.5 was not sensitive to galantamine, Kv1.5 current was not changed by galantamine at concentration of 10 muM. Our data suggest that galantamine potently blocks Kv2.1, but not Kv1.5 channels. (C) 2004 Elsevier B.V. All rights reserved.