Irisin induces white adipose tissue browning in mice as assessed by magnetic resonance imaging

被引:22
作者
Chen, Yue [1 ]
Ding, Jie [1 ]
Zhao, Yufei [1 ]
Ju, Shenghong [1 ]
Mao, Hui [2 ]
Peng, Xin-Gui [1 ]
机构
[1] Southeast Univ, Zhongda Hosp, Med Sch, Jiangsu Key Lab Mol & Funct Imaging,Dept Radiol, Nanjing 210009, Peoples R China
[2] Emory Univ, Dept Radiol & Imaging Sci, Atlanta, GA 30322 USA
基金
中国国家自然科学基金;
关键词
Obesity; adipocytes; white; uncoupling protein 1; metabolic diseases; LIVER FAT-CONTENT; QUANTIFICATION; ADIPOCYTES; CELLS;
D O I
10.1177/15353702211006049
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
This study aimed to track and evaluate the effect of low-dose irisin on the browning of white adipose tissue (WAT) in mice using magnetic resonance imaging (MRI) noninvasively in vivo. Mature white adipocytes extracted from mice were cultured, induced and characterized before being treated by irisin. The volume and fat fraction of WAT were quantified using MRI in normal chow diet and high fat mice after injection of irisin. The browning of cultured white adipocytes and WAT in mice were validated by immunohistochemistry and western blotting for uncoupling protein 1 (UCP1) and deiodinase type II (DIO2). The serum indexes were examined with high fat diet after irisin intervention. UCP1 and DIO2 in adipocytes showed increases responding to the irisin treatment. The size of white adipocytes in mice receiving irisin intervention was reduced. MRI measured volumes and fat fraction of WAT were significantly lower after Irisin treatment. Blood glucose and cholesterol levels were reduced in high fat diet mice after irisin treatment. Irisin intervention exerted browning of WAT, resulting reduction of volume and fat fraction of WAT as measured by MRI. Furthermore, it improved the condition of mice with diet-induced obesity and related metabolic disorders.
引用
收藏
页码:1597 / 1606
页数:10
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