Defensin-mRNA Expression in the Upper Gastrointestinal Tract Is Modulated in Children With Celiac Disease and Helicobacter pylori-positive Gastritis

被引:23
作者
Vordenbaeumen, Stefan [1 ]
Pilic, Denisa [2 ]
Otte, Jan-Michel [3 ]
Schmitz, Frank [3 ]
Schmidt-Choudhury, Anjona [2 ]
机构
[1] Univ Dusseldorf, Dept Endocrinol Diabetol & Rheumatol, Dusseldorf, Germany
[2] Ruhr Univ Bochum, St Josef Hosp, Childrens Univ Hosp Bochum, Dept Pediat Gastroenterol, D-4630 Bochum, Germany
[3] Ruhr Univ Bochum, St Josef Hosp, Dept Internal Med 1, D-4630 Bochum, Germany
关键词
celiac disease; defensins; gastrointestinal tract; Helicobacter pylori; innate immunity; HUMAN BETA-DEFENSINS; PANETH CELLS; ANTIMICROBIAL PEPTIDES; EPITHELIAL-CELLS; INNATE IMMUNITY; LYMPHOCYTIC GASTRITIS; HBD-2; BETA-DEFENSIN-2; CATHELICIDINS; MULTIPLE;
D O I
10.1097/MPG.0b013e3181cd26cd
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Objectives: Defensins are expressed in epithelial cells as cationic peptides with antimicrobial properties. Because of their role as immunologically important effector molecules, their contribution in maintaining a stable microenvironment in the gastrointestinal tract has recently received much attention. The present study was designed to further characterize expression patterns of defensins in diseases of the upper gastrointestinal tract in children, particularly in Helicobacter pylori (Hp)-associated gastritis or celiac disease (CD). Patients and Methods: Semiquantitative real-time reverse transcriptase-polymerase chain reaction (PCR) was carried out with gene-specific primers for human beta-defensin 1 to 6 (hBD1 to 6) and human alpha-defensin 5 and 6 (hD5 and 6) in mucosal biopsies of children diagnosed as having CD (n = 11; 4.2 - 16.2 years) or Hp gastritis (n = 18; 3.2 - 16.7 years). Levels of expression were compared with those of healthy individuals (n = 21; 2.8 - 14.6 years). Expression levels in Hp-infected specimens were furthermore compared with those with histologic inflammation not associated with Hp infection (n = 30; 3.6 - 15.7 years). Results: Expression of hBD2 was upregulated in the antrum and corpus of patients with Hp gastritis, whereas inflammation without detection of Hp was not associated with any change in defensin gene expression. In patients with CD, expression of hBD2 was upregulated in the antrum, whereas hBD1 and 4 were downregulated in duodenal biopsies. Conclusions: Different pathological conditions of the upper gastrointestinal tract lead to specific modulations of defensin gene expression in children. Especially the pathophysiological role of hBD2 in Hp infection and hBD1 and 4 in CD warrant further attention.
引用
收藏
页码:596 / 600
页数:5
相关论文
共 43 条
[1]  
Alp S, 2005, EUR J MED RES, V10, P1
[2]   The role of Paneth cells and their antimicrobial peptides in innate host defense [J].
Ayabe, T ;
Ashida, T ;
Kohgo, Y ;
Kono, T .
TRENDS IN MICROBIOLOGY, 2004, 12 (08) :394-398
[3]   Modulation of host antimicrobial peptide (β-defensins 1 and 2) expression during gastritis [J].
Bajaj-Elliott, M ;
Fedeli, P ;
Smith, GV ;
Domizio, P ;
Maher, L ;
Ali, RS ;
Quinn, AG ;
Farthing, MJG .
GUT, 2002, 51 (03) :356-361
[4]   Epithelial antimicrobial peptides in host defense against infection [J].
Bals R. .
Respiratory Research, 1 (3)
[5]  
Boniotto M, 2003, EUR J HISTOCHEM, V47, P389
[6]   Expression and regulation of antimicrobial peptides in the gastrointestinal tract [J].
Cunliffe, RN ;
Mahida, YR .
JOURNAL OF LEUKOCYTE BIOLOGY, 2004, 75 (01) :49-58
[7]   Distribution, proliferation, and function of Paneth cells in uncomplicated and complicated adult celiac disease [J].
Di Sabatino, Antonio ;
Miceli, Emanuela ;
Dhaliwal, Winnie ;
Biancheri, Paolo ;
Salerno, Raffaele ;
Cantoro, Laura ;
Vanoli, Alessandro ;
De Vincenzi, Massimo ;
Blanco, Camillo Del Vecchio ;
MacDonald, Thomas T. ;
Corazza, Gino R. .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2008, 130 (01) :34-42
[8]   Classification and grading of gastritis - The updated Sydney System [J].
Dixon, MF ;
Genta, RM ;
Yardley, JH ;
Correa, P ;
Batts, KP ;
Dahms, BB ;
Filipe, MI ;
Haggitt, RC ;
Haot, J ;
Hui, PK ;
Lechago, J ;
Lewin, K ;
Offerhaus, JA ;
Price, AB ;
Riddell, RH ;
Sipponen, P ;
Solcia, E ;
Watanabe, H .
AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 1996, 20 (10) :1161-1181
[9]   Innate immune defence in the human gastrointestinal tract [J].
Dommett, R ;
Zilbauer, M ;
George, JT ;
Bajaj-Elliott, M .
MOLECULAR IMMUNOLOGY, 2005, 42 (08) :903-912
[10]  
Drut R, 2004, MED SCI MONITOR, V10, pCR38