Myosin-V stepping kinetics: A molecular model for processivity

被引:335
作者
Rief, M
Rock, RS
Mehta, AD
Mooseker, MS
Cheney, RE
Spudich, JA [1 ]
机构
[1] Univ N Carolina, Dept Cell & Mol Physiol, Chapel Hill, NC 27599 USA
[2] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
[3] Rockefeller Univ, Howard Hughes Med Inst, New York, NY 10021 USA
[4] Stanford Univ, Med Ctr, Dept Biochem, Stanford, CA 94305 USA
关键词
D O I
10.1073/pnas.97.17.9482
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Myosin-V is a molecular motor that moves processively along its actin track. We have used a feedback-enhanced optical trap to examine the stepping kinetics of this movement. By analyzing the distribution of time periods separating discrete approximate to 36-nm mechanical steps, we characterize the number and duration of rate-limiting biochemical transitions preceding each such step. These data show that myosin-V is a tightly coupled motor whose cycle time is limited by ADP release. On the basis of these results, we propose a model for myosin-V processivity.
引用
收藏
页码:9482 / 9486
页数:5
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