Evaluation of the Interactions between Human Serum Albumin (HSA) and Non-Steroidal Anti-Inflammatory (NSAIDs) Drugs by Multiwavelength Molecular Fluorescence, Structural and Computational Analysis

被引：31

作者：

Amezqueta, Susana ^{[1
,2
]}

Beltran, Jose Luis ^{[1
]}

Bolioli, Anna Maria ^{[1
]}

Campos-Vicens, Lluis ^{[3
,4
]}

Luque, Francisco Javier ^{[2
,3
,5
]}

Rafols, Clara ^{[1
,2
]}

机构：

[1] Univ Barcelona, Fac Chem, Dept Chem Engn & Analyt Chem, Marti & Franques 1-11, Barcelona 08028, Spain

[2] Univ Barcelona, Inst Biomed IBUB, Barcelona 08028, Spain

[3] Univ Barcelona, Fac Pharm & Food Sci, Dept Nutr Food Sci & Gastron, Prat de la Riba 171, Santa Coloma De Gramenet 08921, Spain

[4] Pharmacelera, Torre R,4a Planta,Despatx A05, Barcelona 08028, Spain

[5] Univ Barcelona, Inst Theoret & Computat Chem IQTCUB, Barcelona 08028, Spain

来源：

PHARMACEUTICALS | 2021年 / 14卷 / 03期

关键词：

non-steroidal anti-inflammatory drugs; drug-protein interactions; human serum albumin; fluorescence multiwavelength data treatment; fluorescence quenching; molecular modeling;

D O I：

10.3390/ph14030214

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The interaction between drugs and transport proteins, such as albumins, is a key factor in drug bioavailability. One of the techniques commonly used for the evaluation of the drug-protein complex formation is fluorescence. This work studies the interaction of human serum albumin (HSA) with four non-steroidal anti-inflammatory drugs (NSAIDs)-ibuprofen, flurbiprofen, naproxen, and diflunisal-by monitoring the fluorescence quenching when the drug-albumin complex is formed. Two approaches-the double logarithm Stern-Volmer equation and the STAR program-are used to evaluate the binding parameters. The results are analyzed considering the binding properties, determined by using other complementary techniques and the available structural information of albumin complexes with NSAID-related compounds. Finally, this combined analysis has been synergistically used to interpret the binding of flurbiprofen to HSA.

引用

页数：21

共 41 条

[1] Evaluation of the interactions between human serum albumin (HSA) and warfarin or diflunisal by using molecular fluorescence using two approaches [J].