Microarray Analysis of Oligosaccharide-Mediated Multivalent Carbohydrate-Protein Interactions and Their Heterogeneity

被引:13
作者
Gade, Madhuri [1 ]
Alex, Catherine [1 ]
Ben-Arye, Shani Leviatan [2 ]
Monteiro, Joao T. [3 ,4 ]
Yehuda, Sharon [2 ]
Lepenies, Bernd [3 ,4 ]
Padler-Karavani, Vered [2 ]
Kikkeri, Raghavendra [1 ]
机构
[1] Indian Inst Sci Educ & Res, Dr Homi Bhabha Rd, Pune 411008, Maharashtra, India
[2] Tel Aviv Univ, Dept Cell Res & Immunol, George S Wise Fac Life Sci, IL-69978 Tel Aviv, Israel
[3] Univ Vet Med Hannover, Immunol Unit, Bunteweg 17, D-30559 Hannover, Germany
[4] Univ Vet Med Hannover, Res Ctr Emerging Infect & Zoonoses, Bunteweg 17, D-30559 Hannover, Germany
关键词
C-type lectin; heterogeneity; mannose; microarray; oligosaccharides; DEFINED JANUS GLYCODENDRIMERS; GLYCO-GOLD NANOPARTICLES; LECTIN RECOGNITION; CLICK CHEMISTRY; BINDING; SEQUENCE; GLYCOPOLYMERS; HETEROGLYCOCLUSTERS; GLYCOCLUSTERS; RECEPTOR;
D O I
10.1002/cbic.201800037
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
C-type lectin , heterogeneity , mannose , microarray , oligosaccharidesCarbohydrate-protein interactions (CPIs) are involved in a wide range of biological phenomena. Hence, the characterization and presentation of carbohydrate epitopes that closely mimic the natural environment is one of the long-term goals of glycosciences. Inspired by the multivalency, heterogeneity and nature of carbohydrate ligand-mediated interactions, we constructed a combinatorial library of mannose and galactose homo-and hetero-glycodendrons to study CPIs. Microarray analysis of these glycodendrons with a wide range of biologically important plant and animal lectins revealed that oligosaccharide structures and heterogeneity interact with each other to alter binding preferences.
引用
收藏
页码:1170 / 1177
页数:8
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