Comparisons between the structures of HCV and Rep helicases reveal structural similarities between SF1 and SF2 super-families of helicases

被引：96

作者：

Korolev, S

Yao, NH

Lohman, TM

Weber, PC

Waksman, G

机构：

[1] Washington Univ, Sch Med, Dept Biochem & Mol Biophys, St Louis, MO 63110 USA

[2] Schering Plough Corp, Res Inst, Dept Struct Chem, Kenilworth, NJ 07033 USA

来源：

PROTEIN SCIENCE | 1998年 / 7卷 / 03期

关键词：

HCV; helicase; helicase motif; Rep; structure;

D O I：

10.1002/pro.5560070309

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three helicase structures have been determined recently: that of the DNA helicase PcrA, that of the hepatitis C virus RNA helicase, and that of the Escherichia coli DNA helicase Rep. PcrA and Rep belong to the same super-family of helicases (SF1) and are structurally very similar. In contrast, the HCV helicase belongs to a different super-family of helicases, SF2, and shows little sequence homology with the PcrA/Rep helicases. Yet, the HCV helicase is structurally similar to Rep/PcrA, suggesting preservation of structural scaffolds and relationships between helicase motifs across these two super-families. The comparison study presented here also reveals the existence of a new helicase motif in the SF1 family of helicases.

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收藏

页码：605 / 610

页数：6

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