Use of membraneless osmosis for concentration of proteins from molecular-dispersed and colloidal-dispersed solutions (Review)

Theoretical principles, practical realizations, and future trends in the use of the method of protein concentration based on thermodynamic incompatibility of proteins with polysaccharides are reviewed. The relationship between structural features of these biopolymers (molecular weight, rigidity, and conformation of the polysaccharide chain; the nature and ionogenic properties of its functional groups; the type of protein and state of its molecules), as well as major physicochemical parameters (pH, ionic strength, and temperature) and mechanical shift energy of the system, on the one hand, and its phase diagram, on the other hand, are discussed.