Simultaneous variation of temperature and gradient steepness for reversed-phase high-performance liquid chromatography method development - II. The use of further changes in conditions

The preceding paper (Part I) suggests that simply optimizing temperature and gradient steepness will often provide an adequate reversed-phase HPLC separation. In some cases, however, this procedure will prove unsuccessful, and then further method-development experiments (involving change in other separation conditions) will be required. One strategy is to change a variable other than temperature or gradient steepness, followed by re-optimization of the latter two variables. The present paper examines the application of this approach with the aid of computer simulation to several samples. (C) 1998 Elsevier Science B.V.