Desorption Electrospray Ionization Mass Spectrometry Imaging of Proteins Directly from Biological Tissue Sections

被引：103

作者：

Garza, Kyana Y. ^{[1
]}

Feider, Clara L. ^{[1
]}

Klein, Dustin R. ^{[1
]}

Rosenberg, Jake A. ^{[1
]}

Brodbelt, Jennifer S. ^{[1
]}

Eberlin, Livia S. ^{[1
]}

机构：

[1] Univ Texas Austin, Dept Chem, Austin, TX 78712 USA

来源：

ANALYTICAL CHEMISTRY | 2018年 / 90卷 / 13期

基金：

美国国家卫生研究院;

关键词：

BREAST-CANCER; AMBIENT IONIZATION; OVARIAN-CANCER; S100; PROTEINS; MS;

D O I：

10.1021/acs.analchem.8b00967

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Analysis of large biomolecules including proteins has proven challenging using ambient ionization mass spectrometry imaging techniques. Here, we have successfully optimized desorption electrospray ionization mass spectrometry (DESI-MS) to detect intact proteins directly from tissue sections and further integrated DESI-MS to a high field asymmetric waveform ion mobility (FAIMS) device for protein imaging. Optimized DESI-FAIMS-MS parameters were used to image mouse kidney, mouse brain, and human ovarian and breast tissue samples, allowing detection of 11, 16, 14, and 16 proteoforms, respectively. Identification of protein species detected by DESI-MS was performed on-tissue by top-down ultraviolet photodissociation (UVPD) and collision induced dissociation (CID) as well as using tissue extracts by bottom-up CID and top down UVPD. Our results demonstrate that DESI-MS imaging is suitable for the analysis of the distribution of proteins within biological tissue sections.

引用

收藏

页码：7785 / 7789

页数：5

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