Antivenomic Assessment of the Immunological Reactivity of EchiTAb-Plus-ICP, an Antivenom for the Treatment of Snakebite Envenoming in Sub-Saharan Africa

被引:37
作者
Calvete, Juan J. [1 ]
Cid, Pedro [1 ]
Sanz, Libia [1 ]
Segura, Alvaro [2 ]
Villalta, Mauren [2 ]
Herrera, Maria [2 ]
Leon, Guillermo [2 ]
Harrison, Robert [3 ]
Durfa, Nandul [4 ]
Nasidi, Abdusalami [4 ]
Theakston, R. David G. [3 ]
Warrell, David A. [5 ]
Maria Gutierrez, Jose [2 ]
机构
[1] CSIC, Inst Biomed Valencia, Valencia 46010, Spain
[2] Univ Costa Rica, Fac Microbiol, Inst Clodomiro Picado, San Jose, Costa Rica
[3] Univ Liverpool, Liverpool Sch Trop Med, Alistair Reid Venom Res Unit, Liverpool L3 5QA, Merseyside, England
[4] Fed Minist Hlth, Abuja, Nigeria
[5] Univ Oxford, Nuffield Dept Clin Med, Oxford, England
关键词
VENOM METALLOPROTEINASES; PHOSPHOLIPASE A(2); DNA IMMUNIZATION; CARPET VIPER; BITE; PURIFICATION; OCELLATUS; DISINTEGRINS; PITVIPERS; TAXONOMY;
D O I
10.4269/ajtmh.2010.09-0733
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
The immunoreactivity of EchiTAb-Plus-ICP, an antivenom developed for the treatment of snakebite envenoming in sub-Saharan Africa, to venoms of seven Echis and Bids species, was assessed by "antivenomics." This proteomic approach is based on the ability of an antivenom to immunodeplete homologous or heterologous venom proteins. Our results show an extensive cross-reactivity of this antivenom against all Echis and Bids venoms studied, as revealed by the complete immunodepletion of the majority of venom components, including metalloproteinases, serine proteinases, C-type lectin-like proteins, some phospholipases A(2) and L-amino acid oxidase. However, some phospholipases A(2), disintegrins and proteinase inhibitors were immunodepleted to only a partial extent. These results support the hypothesis that immunizing horses with a mixture of the venoms of Echis ocellatus, Bitis arietans, and Naja nigricollis generates antibodies capable of recognizing the majority of components of medically-relevant homologous and heterologous viperid venoms of the genera Bitis and Echis from sub-Saharan Africa.
引用
收藏
页码:1194 / 1201
页数:8
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