Trypanosoma cruzi amino acid transporter TcAAAP411 mediates arginine uptake in yeasts

被引：22

作者：

Carrillo, Carolina ^{[1
,2
]}

Canepa, Gaspar E. ^{[2
,3
]}

Giacometti, Alina ^{[2
,3
]}

Bouvier, Leon A. ^{[2
,3
]}

Miranda, Mariana R. ^{[2
,3
]}

de los Milagros Camara, Maria ^{[2
,3
]}

Pereira, Claudio A. ^{[2
,3
]}

机构：

[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Fdn Inst Leloir, Buenos Aires, DF, Argentina

[2] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina

[3] Univ Buenos Aires, LBMTC, Dept Sustancias Vasoact, Inst Invest Med Alfredo Lanari, Buenos Aires, DF, Argentina

来源：

FEMS MICROBIOLOGY LETTERS | 2010年 / 306卷 / 02期

关键词：

Trypanosoma cruzi; arginine permease; amino acid transport; AAAP family; Chagas' disease; SACCHAROMYCES-CEREVISIAE; PROTOZOAN PARASITES; LEISHMANIA-DONOVANI; KINASE; PERMEASES; AFRICAN; FAMILY;

D O I：

10.1111/j.1574-6968.2010.01936.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Trypanosoma cruzi, the aetiological agent of Chagas' disease, is exposed to extremely different environment conditions during its life cycle, and transporters are key molecules for its adaptive regulation. Amino acids, and particularly arginine, are essential components in T. cruzi metabolism. In this work, a novel T. cruzi arginine permease was identified by screening different members of the AAAP family (amino acid/auxin permeases) in yeast complementation assays using a toxic arginine analogue. One gene candidate, TcAAAP411, was characterized as a very specific, high-affinity, l-arginine permease. This work is the first identification of the molecular components involved specifically in amino acid transport in T. cruzi and provides new insights for further validation of the TcAAAP family as functional permeases.

引用

页码：97 / 102

页数：6

共 26 条

[1] Novel motifs in amino acid permease genes from Leishmania [J].

Akerman, M ;

Shaked-Mishan, P ;

Mazareb, S ;

Volpin, H ;

Zilberstein, D .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 325 (01) :353-366

[2] Arginine kinase of the flagellate protozoa Trypanosoma cruzi -: Regulation of its expression and catalytic activity [J].

Alonso, GD ;

Pereira, CA ;

Remedi, MS ;

Paveto, MC ;

Cochella, L ;

Ivaldi, MS ;

de Burgos, NMG ;

Torres, HN ;

Flawiá, MM .

FEBS LETTERS, 2001, 498 (01) :22-25

[3] Targeting of toxic compounds to the trypanosome's interior [J].

Barrett, Michael P. ;

Gilbert, Ian H. .

ADVANCES IN PARASITOLOGY, VOL 63, 2006, 63 :125-183

[4] The genome of the African trypanosome Trypanosoma brucei [J].

Berriman, M ;

Ghedin, E ;

Hertz-Fowler, C ;

Blandin, G ;

Renauld, H ;

Bartholomeu, DC ;

Lennard, NJ ;

Caler, E ;

Hamlin, NE ;

Haas, B ;

Böhme, W ;

Hannick, L ;

Aslett, MA ;

Shallom, J ;

Marcello, L ;

Hou, LH ;

Wickstead, B ;

Alsmark, UCM ;

Arrowsmith, C ;

Atkin, RJ ;

Barron, AJ ;

Bringaud, F ;

Brooks, K ;

Carrington, M ;

Cherevach, I ;

Chillingworth, TJ ;

Churcher, C ;

Clark, LN ;

Corton, CH ;

Cronin, A ;

Davies, RM ;

Doggett, J ;

Djikeng, A ;

Feldblyum, T ;

Field, MC ;

Fraser, A ;

Goodhead, I ;

Hance, Z ;

Harper, D ;

Harris, BR ;

Hauser, H ;

Hostetter, J ;

Ivens, A ;

Jagels, K ;

Johnson, D ;

Johnson, J ;

Jones, K ;

Kerhornou, AX ;

Koo, H ;

Larke, N .

SCIENCE, 2005, 309 (5733) :416-422

[5] Post genomic analysis of permeases from the amino acid/auxin family in protozoan parasites [J].

Bouvier, LA ;

Silber, AM ;

Lopes, CG ;

Canepa, GE ;

Miranda, MR ;

Tonelli, RR ;

Colli, W ;

Alves, MJM ;

Pereira, CA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 321 (03) :547-556

[6] BIOLOGY OF TRYPANOSOMA-CRUZI [J].

BRENER, Z .

ANNUAL REVIEW OF MICROBIOLOGY, 1973, 27 :347-382

[7]

Canepa GE, 2004, FEMS MICROBIOL LETT, V236, P79, DOI 10.1111/j.1574-6968.2004.tb09630.x

[8] Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi [J].

Carrillo, Carolina ;

Canepa, Gaspar E. ;

Algranati, Israel D. ;

Pereira, Claudio A. .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 344 (03) :936-940

[9] AMMONIA REGULATION OF AMINO-ACID PERMEASES IN SACCHAROMYCES-CEREVISIAE [J].

COURCHESNE, WE ;

MAGASANIK, B .

MOLECULAR AND CELLULAR BIOLOGY, 1983, 3 (04) :672-683

[10] Arginine Homeostasis and Transport in the Human Pathogen Leishmania donovani [J].

Darlyuk, Ilona ;

Goldman, Adele ;

Roberts, Sigrid C. ;

Ullman, Buddy ;

Rentsch, Doris ;

Zilberstein, Dan .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (30) :19800-19807

← 1 2 3 →