Cloning and characterization of a corepressor and potential component of the nuclear hormone receptor repression complex

被引：106
作者：
Zamir, I
Dawson, J
Lavinsky, RM
Glass, CK
Rosenfeld, MG
Lazar, MA
机构：
[1] Univ Penn, Sch Med, Dept Med, Div Endocrinol Diabet & Metab, Philadelphia, PA 19104 USA
[2] Univ Penn, Sch Med, Dept Genet, Philadelphia, PA 19104 USA
[3] Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92093 USA
[4] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA
[5] Univ Calif San Diego, Sch Med, La Jolla, CA 92093 USA
[6] Univ Calif San Diego, Grad Program Biol, La Jolla, CA 92093 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 26期
关键词：
D O I：
10.1073/pnas.94.26.14400
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Nuclear hormone receptors are potent repressors of transcription in the unliganded state, We describe here the cloning of a nuclear receptor corepressor that we call SUN-CoR (Small Unique Nuclear receptor CoRepressor), which shows no homology to previously described nuclear hormone receptor corepressors, N-CoR or SMRT, SUN-CoR is a highly basic, 16-kDa nuclear protein that is expressed at high levels in adult tissues and is induced during adipocyte and myogenic differentiation. SUN-CoR potentiates transcriptional repression by thyroid hormone receptor and RevErb in vivo, represses transcription when fused to a heterologous DNA binding domain, and interacts with RevErb as well as with thyroid hormone receptor in vitro, SUN-CoR also interacts with N-CoR and SMRT in vitro and with endogenous N-CoR in cells, We conclude that SUN-CoR is a corepressor and may function as an additional component of the complex involved in transcriptional repression by unliganded and orphan nuclear hormone receptors.
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页码：14400 / 14405
页数：6
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