Synthesis of oligogalacturonates conjugated to BSA

被引：23

作者：

Clausen, MH ^{[1
]}

Madsen, R ^{[1
]}

机构：

[1] Tech Univ Denmark, Dept Chem, DK-2800 Lyngby, Denmark

来源：

CARBOHYDRATE RESEARCH | 2004年 / 339卷 / 13期

关键词：

antigen; galactose; neoglycoprotein; pectin; pentenyl glycoside;

D O I：

10.1016/j.carres.2004.06.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of three oligogalacturonates with an aldehyde spacer attached at the reducing end is described. Trigalacturonates alpha-D-GalpA-(1-->4)-alpha-D-GalpA-(1-->4)-alpha-D-GalpA-(1-->O(CH2)(7)CHO and alpha-D-GalpA(Me)-(1-->4)-alpha-D-GalpA(Me)(1-->4)-alpha-D-GalpA(Me)-(1--> O(CH2)(7)CHO as well as hexagalacturonate alpha-D-GalpA-(1-->4)-[alpha-D-GalpA-(14)](4)-alpha-D-GalpA(1-->O(CH2)(7)CHO are prepared by stepwise coupling of galactose units followed by oxidation of the 6-positions. The alpha-linkages are formed by employing n-pentenyl galactosides as glycosyl donors and N-iodosuccinimide/triethylsilyl triflate as the promoter. Deprotection furnishes the three target oligogalacturonates, which are subsequently linked to bovine serum albumin by reductive amination. These neoglycoproteins will serve as immunogens for generation of new antibodies that can be used for localization and characterization of pectin in plants. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：2159 / 2169

页数：11

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