Tagging STMS markers to Fusarium wilt race-1 resistance in chickpea (Cicer arietinum L.)

被引:0
|
作者
Barman, Pranjan [1 ]
Handique, A. K. [1 ]
Tanti, Bhaben [2 ]
机构
[1] Gauhati Univ, Dept Biotechnol, Gauhati 781014, India
[2] Gauhati Univ, Dept Bot, Gauhati 781014, India
来源
INDIAN JOURNAL OF BIOTECHNOLOGY | 2014年 / 13卷 / 03期
关键词
Chickpea; Fusarium wilt; Fusarium oxysporum; STMS; tagging; linkage map; LINKAGE MAP; GENE; POPULATIONS; INHERITANCE;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Fusarium wilt in chickpea (Cicer arietinum L.) is caused by fungal pathogen Fusarium oxysporum f. sp. ciceris. Eight races of Fusarium have been reported worldwide. In India, races 1 and 2 are the most predominant and cause widespread damage, resulting up to 70% yield loss. Efforts are on to tag molecular markers to loci responsible for resistance against various races of the pathogen. Here we report tagging of STMS (sequence-tagged microsatellite site) markers with race-1 resistance for Fusarium wilt (foc-1). A linkage map was generated using the scored markers on 82 RILs (recombinant inbred lines) of a cross WR-315 x C-104 along with the phenotypic marker. The most closely linked marker, TA37 was identified at a distance of 0.2 cM from foc-1 in linkage group 2 (LG2). Additionally, 2 more markers TA200 and TR2 were also identified at a distance of 1.0 and 3.0 cM, respectively, flanking the foc-1 locus. Markers TA37, TA200 and TR2 can be used in marker assisted selection, introgression of resistance gene (R-gene) into economically important cultivars and finally to clone the candidate gene for Fusarium-wilt resistance.
引用
收藏
页码:370 / 375
页数:6
相关论文
empty
未找到相关数据