A novel automatable enzyme-coupled colorimetric assay for endo-1,4-β-glucanase (cellulase)

被引：15

作者：

Mangan, David ^{[1
]}

Cornaggia, Claudio ^{[1
]}

McKie, Vincent ^{[1
]}

Kargelis, Tadas ^{[1
]}

McCleary, Barry V. ^{[1
]}

机构：

[1] Megazyme Int Ireland, Bray Business Pk,Southern Cross Rd, Bray A98 YV29, County Wicklow, Ireland

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2016年 / 408卷 / 15期

关键词：

endo-1,4-beta-Glucanase; Cellulase; Assay; Colorimetric; CELLG5; Automation; ALKALINE CELLULASE; SPECIFICITY; ADAPTATION; SUBSTRATE;

D O I：

10.1007/s00216-016-9507-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

endo-1,4-beta-Glucanase (endo-cellulase, EC 3.2.1.4) is one of the most widely used enzymes in industry. Despite its importance, improved methods for the rapid, selective, quantitative assay of this enzyme have been slow to emerge. In 2014, a novel enzyme-coupled assay that addressed many of the limitations of the existing assay methodology was reported. This involved the use of a bifunctional substrate chemically derived from cellotriose. Reported herein is a much improved version of this assay employing a novel substrate, namely 4,6-O-(3-ketobutylidene)-4-nitrophenyl-beta-d-cellopentaoside.

引用

页码：4159 / 4168

页数：10

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