cAMP-GEFII is a direct target of cAMP in regulated exocytosis

被引：391

作者：

Ozaki, N ^{[1
]}

Shibasaki, T

Kashima, Y

Miki, T

Takahashi, K

Ueno, H

Sunaga, Y

Yano, H

Matsuura, Y

Iwanaga, T

Takai, Y

Seino, S

机构：

[1] Chiba Univ, Grad Sch Med, Dept Mol Med, Chiba 2608670, Japan

[2] Chiba Univ, Grad Sch Med, Dept Mol Immunol, Chiba 2608670, Japan

[3] Natl Inst Infect Dis, Dept Virol 2, Tokyo 1628640, Japan

[4] Hokkaido Univ, Grad Sch Vet Med, Lab Anat, Sapporo, Hokkaido 0600818, Japan

[5] Osaka Univ, Grad Sch Med, Fac Med, Dept Mol Biol & Biochem, Suita, Osaka 5650871, Japan

来源：

NATURE CELL BIOLOGY | 2000年 / 2卷 / 11期

基金：

日本学术振兴会;

关键词：

D O I：

10.1038/35041046

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Although cAMP is well known to regulate exocytosis in many secretory cells, its direct target in the exocytotic machinery is not known. Here we show that cAMP-GEFII, a cAMP sensor, binds to Rim (Rab3-interacting molecule, Rab3 being a small G protein) and to a new isoform, RimP, both of which are putative regulators of fusion of vesicles to the plasma membrane. We also show that cAMP-GEFII, through its interaction with Rim2, mediates cAMP-induced, Ca2+-dependent secretion that is not blocked by an inhibitor of cAMP-dependent protein kinase (PKA). Accordingly, cAMP-GEFII is a direct target of cAMP in regulated exocytosis and is responsible for cAMP-dependent, PKA-independent exocytosis.

引用

页码：805 / 811

页数：7

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