Thyroid oxidase (THOX2) gene expression in the rat thyroid cell line FRTL-5

被引：54
|
作者：
Dupuy, C [1 ]
Pomerance, M [1 ]
Ohayon, R [1 ]
Noël-Hudson, MS [1 ]
Déme, D [1 ]
Chaaraoui, M [1 ]
Francon, J [1 ]
Virion, A [1 ]
机构：
[1] Fac Pharm Chatenay Malabry, INSERM, U486, F-92296 Chatenay Malabry, France
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 277卷 / 02期
关键词：
D O I：
10.1006/bbrc.2000.3671
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A cDNA encoding an NADPH oxidase flavoprotein was isolated from the rat thyroid gland. The predicted 1517-residue polypeptide was 82.5% identical to the human THOX2/DUOX2 and 74% similar to THOX1/DUOX1, Rat THOX2 lacks a stretch of 30 residues, corresponding to one exon in the human gene sequence. THOX2 mRNA was found to be expressed in cultured FRTL-5 cells. The level of THOX2 mRNA was increased by cAMP in these cells and it was decreased in the thyroids of rats treated with the antithyroid drug methimazole, unlike the TPO and NIS mRNAs. Since it was found in the intestine, duodenum, and colon, in addition to thyroid, we suggest that it be called LNOX, the new family of lo ng homologs of NOX flavoproteins rather than THOX and/or DUOX (C) 2000 Academic Press.
引用
收藏
页码：287 / 292
页数：6
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