Stimulation of synaptosomal D-[3H]aspartate transport by substance P in rat brain

被引：3

作者：

Healy, J ^{[1
]}

Downes, A ^{[1
]}

McBean, GJ ^{[1
]}

机构：

[1] Univ Coll Dublin, Dept Biochem, Dublin 4, Ireland

来源：

NEUROSCIENCE LETTERS | 2000年 / 290卷 / 02期

关键词：

substance P; glutamate; D-aspartate; transport; synaptosomes; NK1; receptors;

D O I：

10.1016/S0304-3940(00)01318-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effect of the neuropeptide, substance P, on the transport of D-[H-3]aspartate into rat striatal synaptosomes was studied. Almost 90% of the total transport of D-[H-3]aspartate was sodium-dependent and the maximum rate (V-max) of this transport was increased by 34% of control by 2.5 nM substance P (EC50 = 0.52 nM). In contrast, sodium-independent transport was inhibited by substance P, The N K-1 antagonist, L706303 (500 nM) blocked the stimulation of D-[H-3]aspartate transport by 2.5 nM substance P, but did not a Iter D-aspartate uptake in the absence of substance P. These results indicate that high affinity glutamate transporters in the brain may be under positive regulation by substance P, and suggest a previously-unidentified mechanism of control of glutamate transport. (C) 2000 Elsevier Science Ireland Ltd, All rights reserved.

引用

页码：113 / 116

页数：4

共 18 条

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[2]

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