The role of gut microbiota in health and disease: In vitro modeling of host-microbe interactions at the aerobe-anaerobe interphase of the human gut

被引：133

作者：

von Martels, Julius Z. H. ^{[1
]}

Sadabad, Mehdi Sadaghian ^{[2
]}

Bourgonje, Arno R. ^{[1
]}

Blokzijl, Tjasso ^{[1
]}

Dijkstra, Gerard ^{[1
]}

Faber, Klaas Nico ^{[1
]}

Harmsen, Hermie J. M. ^{[2
]}

机构：

[1] Univ Groningen, Univ Med Ctr Groningen, Dept Gastroenterol & Hepatol, Groningen, Netherlands

[2] Univ Groningen, Univ Med Ctr Groningen, Dept Med Microbiol, Groningen, Netherlands

来源：

ANAEROBE | 2017年 / 44卷

关键词：

Anaerobic gut bacteria; Human intestinal epithelium; Co-culture system; Host-microbe interactions; Transwell co-culture; Host-Microbiota Interaction (HMI) module; Human oxygen-Bacteria anaerobic; (HoxBan) system; The human gut-on-a-chip; HuMiX model; HUMAN GASTROINTESTINAL-TRACT; INFLAMMATORY-BOWEL-DISEASE; FAECALIBACTERIUM-PRAUSNITZII; INTESTINAL MICROBIOTA; TRANSCRIPTOME RESPONSES; MAINTENANCE TREATMENT; COLONIC FERMENTATION; CELIAC-DISEASE; ORGAN-CULTURE; BACTERIA;

D O I：

10.1016/j.anaerobe.2017.01.001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The microbiota of the gut has many crucial functions in human health. Dysbiosis of the microbiota has been correlated to a large and still increasing number of diseases. Recent studies have mostly focused on analyzing the associations between disease and an aberrant microbiota composition. Functional studies using (in vitro) gut models are required to investigate the precise interactions that occur between specific bacteria (or bacterial mixtures) and gut epithelial cells. As most gut bacteria are obligate or facultative anaerobes, studying their effect on oxygen-requiring human gut epithelial cells is technically challenging. Still, several (anaerobic) bacterial-epithelial co-culture systems have recently been developed that mimic host-microbe interactions occurring in the human gut, including 1) the Transwell "apical anaerobic model of the intestinal epithelial barrier", 2) the Host-Microbiota Interaction (HMI) module, 3) the "Human oxygen-Bacteria anaerobic" (HoxBan) system, 4) the human gut-on-a-chip and 5) the HuMiX model. This review discusses the role of gut microbiota in health and disease and gives an overview of the characteristics and applications of these novel host-microbe co-culture systems. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：3 / 12

页数：10

共 131 条

[1] In Vitro Characterization of the Impact of Different Substrates on Metabolite Production, Energy Extraction and Composition of Gut Microbiota from Lean and Obese Subjects [J].

Aguirre, Marisol ;

Jonkers, Daisy M. A. E. ;

Troost, Freddy J. ;

Roeselers, Guus ;

Venema, Koen .

PLOS ONE, 2014, 9 (11)

[2] The effect of probiotic strains on the microbiota of the Simulator of the Human Intestinal Microbial EcoSystem (SHIME) [J].

Alander, M ;

De Smet, I ;

Nollet, L ;

Verstraete, W ;

von Wright, A ;

Mattila-Sandholm, T .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1999, 46 (01) :71-79

[3] Enterotypes of the human gut microbiome [J].

Arumugam, Manimozhiyan ;

Raes, Jeroen ;

Pelletier, Eric ;

Le Paslier, Denis ;

Yamada, Takuji ;

Mende, Daniel R. ;

Fernandes, Gabriel R. ;

Tap, Julien ;

Bruls, Thomas ;

Batto, Jean-Michel ;

Bertalan, Marcelo ;

Borruel, Natalia ;

Casellas, Francesc ;

Fernandez, Leyden ;

Gautier, Laurent ;

Hansen, Torben ;

Hattori, Masahira ;

Hayashi, Tetsuya ;

Kleerebezem, Michiel ;

Kurokawa, Ken ;

Leclerc, Marion ;

Levenez, Florence ;

Manichanh, Chaysavanh ;

Nielsen, H. Bjorn ;

Nielsen, Trine ;

Pons, Nicolas ;

Poulain, Julie ;

Qin, Junjie ;

Sicheritz-Ponten, Thomas ;

Tims, Sebastian ;

Torrents, David ;

Ugarte, Edgardo ;

Zoetendal, Erwin G. ;

Wang, Jun ;

Guarner, Francisco ;

Pedersen, Oluf ;

de Vos, Willem M. ;

Brunak, Soren ;

Dore, Joel ;

Weissenbach, Jean ;

Ehrlich, S. Dusko ;

Bork, Peer .

NATURE, 2011, 473 (7346) :174-180

[4] Host-bacterial mutualism in the human intestine [J].

Bäckhed, F ;

Ley, RE ;

Sonnenburg, JL ;

Peterson, DA ;

Gordon, JI .

SCIENCE, 2005, 307 (5717) :1915-1920

[5] Host-bacteria interactions in the intestine: homeostasis to chronic inflammation [J].

Barbosa, Theolis ;

Rescigno, Maria .

WILEY INTERDISCIPLINARY REVIEWS-SYSTEMS BIOLOGY AND MEDICINE, 2010, 2 (01) :80-97

[6] Randomised, double-blind, placebo-controlled trial of fructo-oligosaccharides in active Crohn's disease [J].

Benjamin, Jane L. ;

Hedin, Charlotte R. H. ;

Koutsoumpas, Andreas ;

Ng, Siew C. ;

McCarthy, Neil E. ;

Hart, Ailsa L. ;

Kamm, Michael A. ;

Sanderson, Jeremy D. ;

Knight, Stella C. ;

Forbes, Alastair ;

Stagg, Andrew J. ;

Whelan, Kevin ;

Lindsay, James O. .

GUT, 2011, 60 (07) :923-929

[7] Impact of digestive and oropharyngeal decontamination on the intestinal microbiota in ICU patients [J].

Benus, Robin F. ;

Harmsen, Hermie J. ;

Welling, Gjalt W. ;

Spanjersberg, Rob ;

Zijlstra, Jan G. ;

Degener, John E. ;

van der Werf, Tjip S. .

INTENSIVE CARE MEDICINE, 2010, 36 (08) :1394-1402

[8] Association between Faecalibacterium prausnitzii and dietary fibre in colonic fermentation in healthy human subjects [J].

Benus, Robin F. J. ;

van der Werf, Tjip S. ;

Welling, Gjalt W. ;

Judd, Patricia A. ;

Taylor, Moira A. ;

Harmsen, Hermie J. M. ;

Whelan, Kevin .

BRITISH JOURNAL OF NUTRITION, 2010, 104 (05) :693-700

[9] Culturing of 'unculturable' human microbiota reveals novel taxa and extensive sporulation [J].

Browne, Hilary P. ;

Forster, Samuel C. ;

Anonye, Blessing O. ;

Kumar, Nitin ;

Neville, B. Anne ;

Stares, Mark D. ;

Goulding, David ;

Lawley, Trevor D. .

NATURE, 2016, 533 (7604) :543-+

[10] ORGAN CULTURE OF MUCOSAL BIOPSIES OF HUMAN SMALL INTESTINE [J].

BROWNING, TH ;

TRIER, JS .

JOURNAL OF CLINICAL INVESTIGATION, 1969, 48 (08) :1423-+

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