Three-dimensional biomolecule patterning

被引：18
作者：
Farsari, Maria
Filippidis, George
Drakakis, Theodore S.
Sambani, Kyriaki
Georgiou, Savas
Papadakis, George
Gizeli, Electra
Fotakis, Costas
机构：
[1] Fdn Res & Technol Hellas, IESL, Iraklion 71110, Crete, Greece
[2] Univ Crete, Dept Phys, Iraklion, Greece
[3] Univ Crete, Dept Biol, Iraklion, Greece
来源：
APPLIED SURFACE SCIENCE | 2007年 / 253卷 / 19期
关键词：
photopolymerization; biotin; nonlinear stereolithography;
D O I：
10.1016/j.apsusc.2007.02.177
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
A new method is demonstrated, where three-dimensional protein structures are made by employing multi-photon polymerization and photobiotin photolysis. The technique enables the construction of arbitrary two- and three-dimensional shapes with submicron resolution. The integrity of the immobilized biotin is confirmed by derivatization with fluorescently labeled streptavidin. Fluorescence microscopy is used in order to visualize the distribution of fluorescent streptavidin on the 3D structure. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：8115 / 8118
页数：4
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