Electrochemical nucleic acid-based biosensors: Concepts, terms, and methodology (IUPAC Technical Report)

被引：185

作者：

Labuda, Jan ^{[1
]}

Oliveira Brett, Ana Maria ^{[2
]}

Evtugyn, Gennady ^{[3
]}

Fojta, Miroslav ^{[4
]}

Mascini, Marco ^{[5
]}

Ozsoz, Mehmet ^{[6
]}

Palchetti, Ilaria ^{[5
]}

Palecek, Emil ^{[4
]}

Wang, Joseph ^{[7
]}

机构：

[1] Slovak Univ Technol Bratislava, Fac Chem & Food Technol, Inst Analyt Chem, Bratislava 81237, Slovakia

[2] Univ Coimbra, Fac Sci & Technol, Dept Chem, P-3004533 Coimbra, Portugal

[3] Kazan VI Lenin State Univ, AM Butlerov Inst Chem, Dept Analyt Chem, Kazan 420008, Russia

[4] Acad Sci Czech Republ, Inst Biophys, CS-61265 Brno, Czech Republic

[5] Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy

[6] Ege Univ, Fac Pharm, TR-35100 Bornova, Turkey

[7] Univ San Diego, Dept Nanoengn, La Jolla, CA 92093 USA

来源：

PURE AND APPLIED CHEMISTRY | 2010年 / 82卷 / 05期

关键词：

aptamers; biosensors; electrochemistry; DNA damage; IUPAC Analytical Chemistry Division; nucleic acids; nucleic acid hybridization; nucleic acid interactions; SOLID AMALGAM ELECTRODES; DNA HYBRIDIZATION; IMMOBILIZATION; SENSORS; PROBES;

D O I：

10.1351/PAC-REP-09-08-16

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An electrochemical nucleic acid (NA)-based biosensor is a biosensor that integrates a nucleic acid as the biological recognition element and an electrode as the electrochemical signal transducer. The present report provides concepts, terms, and methodology related to biorecognition elements, detection principles, type of interactions to be addressed, and construction and performance of electrochemical NA biosensors, including their critical evaluation, which should be valuable for a wide audience, from academic, biomedical, environmental, and food-testing, drug-developing, etc. laboratories to sensor producers.

引用

页码：1161 / 1187

页数：27

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