Biosynthesis and characterization of gold nanoparticles: Kinetics, in vitro and in vivo study

被引:76
作者
Ahmad, Nabeel [1 ]
Bhatnagar, Sharad [1 ,2 ]
Saxena, Ritika [1 ]
Iqbal, Danish [1 ,3 ]
Ghosh, A. K. [4 ]
Dutta, Rajiv [5 ]
机构
[1] IFTM Univ, Sch Engn & Technol, Dept Biotechnol, Moradabad, UP, India
[2] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki, Japan
[3] Majmaah Univ, Dept Med Lab Sci, Coll Appl Med Sci, Al Majmaah 11952, Saudi Arabia
[4] IFTM Univ, Sch Pharmaceut Sci, Moradabad, UP, India
[5] Sharda Univ, Dept Biotechnol, Plot 32 & 34,Knowledge Pk 3, Greater Noida 201306, UP, India
来源
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2017年 / 78卷
关键词
Gold nanoparticles; Green synthesis; Kinetics; Cancerous cell lines; Anti-inflammatory; Analgesic animal model; SILVER NANOPARTICLES; BIOLOGICAL SYNTHESIS; AQUEOUS EXTRACT; ROUTE;
D O I
10.1016/j.msec.2017.03.282
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
This study reports a facile, cost effective, nontoxic and eco-friendly method for the synthesis of gold nanoparticles. In this paper, leaf extract of Mentha piperita was successfully used to reduce chloroauric acid, leading to synthesis of gold nanoparticles (AuNPs). The synthesized nanoparticles were further characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy, dynamic light scattering, transmission electron microscopy and field emission scanning electron microscopy. Kinetics studies like effect of volume of leaf extract, precursor, pH, temperature for the synthesis of AuNPs were studied spectrophotometrically. Synthesized AuNPs were found to possess hexagon structure where size of nanoparticles was similar to 78 nm in diameter. These biologically synthesized AuNPs exhibited significant activity against cancerous cell lines MDA-MB-231 and A549 and was compared with the normal 3T3-L1 cell line. Anti-inflammatory and analgesic activities were studied on a Wistar rat model to gauge the impact of AuNPs for a probable role in these applications. AuNPs gave positive results for both these activities, although the potency was less as compared to the standard drugs. These results suggested that the leaves extract of Mentha piperita is a very good bioreductant for the synthesis of AuNPs and have potential for various biomedical and pharmaceutical applications. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:553 / 564
页数:12
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