Responses of the antioxidant defenses of the Goldfish Carassius auratus, exposed to 2,4-dichlorophenol

Goldfish Carassius auratus were exposed to 0.1 mg/l of 2,4-dichlorophenol (2,4-DCP), widely used as transportation power in China. for 2. 5, 10, 20 and 40 days, while one control group was designated for each exposure group. Antioxidant defenses consisting of contents of reduced glutathione (GSH) and glutathione disulfide (GSSG) and activities of superoxide dismutase (SOD). catalase (CAT). glutathione reductase (GR), selenium-dependent glutathione peroxidase (Se-GPx) and glutathione S-transferase (GST) in liver of freshwater fish were determined and the GSH-GSSG ratio and content of tGSH (total glutathione) were calculated. In the present study, the role of hepatic antioxidant defenses was evaluated and the possible poisoning mechanism of fish can be explained as an oxidative stress mechanism. In addition. hepatic SOD and GSH, especially tGSH, were sensitive to 2.4-DCP contamination and thus, can possibly be used in early assessment of 2.4-DCP-dominant polluted aquatic ecosystems. (C) 2004 Elsevier B.V. All rights reserved.