Nox4-dependent ROS production is involved in CVB3-induced myocardial apoptosis

被引：23
作者：
Chi, Jinyu [1 ]
Yu, Shouxian [1 ]
Liu, Chunnan [1 ]
Zhao, Xiaoyu [1 ]
Zhong, Jiaoyue [1 ]
Liang, Yuting [1 ]
Ta, Na [1 ]
Yin, Xinhua [1 ]
Zhao, Dechao [1 ]
机构：
[1] Harbin Med Univ, Affiliated Hosp 1, Dept Cardiol, 199 Dazhi St, Harbin 150001, Heilongjiang, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 503卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Viral myocarditis; CVB3; Nox4; ROS; Apoptosis; VIRAL MYOCARDITIS; CANCER CELLS; PATHWAY;
D O I：
10.1016/j.bbrc.2018.07.093
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Viral myocarditis is a cardiovascular disease that seriously affects human health. Its mechanism is not clear. Coxsackievirus B3 (CVB3) is a member of the picornavirus family and is the leading cause of viral myocarditis. Our group tested the genes in a mouse model of CVB3 virus infection and confirmed that the NADPH oxidase gene had a high expression trend in the acute phase of infection. Whether Nox4, the homologue of NADPH oxidase, participates in the process of viral myocarditis has not been reported. In this study, we found increased expression of Nox4 in viral myocarditis in vivo and in vitro. DPI is a nonspecific inhibitor of Nox4 that improved CVB3-induced myocarditis after injection in vivo. DPI also inhibited intracellular ROS release and apoptosis in vitro. Our data indicated that Nox4-dependent ROS production was involved in CVB3-induced myocardial apoptosis. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码：1641 / 1644
页数：4
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