Dual Site-Specific Labeling of an Antibody Fragment through Sortase A and π-Clamp Conjugation

被引：20

作者：

Lee, Michael D. ^{[1
]}

Tong, Wing Yin ^{[2
]}

Nebl, Thomas ^{[1
]}

Pearce, Lesley A. ^{[1
]}

Pham, Tam M. ^{[1
]}

Golbaz-Hagh, Arghavan ^{[3
]}

Puttick, Simon ^{[3
,4
]}

Rose, Stephen ^{[4
]}

Adams, Timothy E. ^{[1
]}

Williams, Charlotte C. ^{[1
]}

机构：

[1] CSIRO Mfg, Parkville, Vic 3052, Australia

[2] Monash Univ, Monash Inst Pharmaceut Sci, Parkville, Vic 3052, Australia

[3] Univ Queensland, Australian Inst Bioengn & Nanotechnol, St Lucia, Qld 4072, Australia

[4] CSIRO Hlth & Biosecur, Herston, Qld 4029, Australia

来源：

BIOCONJUGATE CHEMISTRY | 2019年 / 30卷 / 10期

关键词：

CYSTEINE; DRUG;

D O I：

10.1021/acs.bioconjchem.9b00639

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The functionalization of proteins with different cargo molecules is highly desirable for a broad range of applications. However, the reproducible production of defined conjugates with multiple functionalities is a significant challenge. Herein, we report the dual site-specific labeling of an antibody fragment, utilizing the orthogonal Sortase A and pi-clamp conjugation methods, and demonstrate that binding of the antibody fragment to its target receptor is retained after dual labeling.

引用

收藏

页码：2539 / 2543

页数：5

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