Regulation of gene expression and biological activity of rainbow trout estrogen receptor

被引：33

作者：

Pakdel, F ^{[1
]}

Delaunay, F ^{[1
]}

Ducouret, B ^{[1
]}

Flouriot, G ^{[1
]}

Kern, L ^{[1
]}

Lazennec, G ^{[1
]}

Le Drean, Y ^{[1
]}

Petit, F ^{[1
]}

Salbert, G ^{[1
]}

Saligaut, D ^{[1
]}

Tujague, M ^{[1
]}

Valotaire, Y ^{[1
]}

机构：

[1] Univ Rennes 1, INRA,Equipe Associee Endocrinol Mol Poissons, URA CNRS 256, France Inst Rennais Biol & Ecol Poissons, Rennes, France

来源：

FISH PHYSIOLOGY AND BIOCHEMISTRY | 1997年 / 17卷 / 1-6期

关键词：

reproduction; steroid receptors; estradiol; estrogen receptor; vitellogenin; liver; hepatocyte; gene transcription; mRNA stability; fish; rainbow trout;

D O I：

10.1023/A:1007706207857

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rainbow trout estrogen receptor (rtER) concentration was highly induced in the liver after in vivo estradiol (E-2) treatment or in vitro, in hepatocyte aggregate culture. Determination of transcription rate and mRNA half-life demonstrated that E-2-induction of hepatic rtER level is caused essentially by an increase in the transcriptional and post-transcriptional activity of rtER gene. However, the expression of rtER gene in the liver seems to be down-regulated by glucocorticoids. We have used transient transfection assays with reporter plasmids linked to 5' flanking regions of the rtER gene promoter, to identify cis-elements responsible for E-2 inducibility. Deletion analysis localized a functional estrogen-responsive-element (ERE), near the transcription start site, with one mutation on the first base compared to the consensus sequence. This element and 200 bp fragment of the rtER promoter encompassing the ERE appear to be the major cis-acting element involved in the regulation of the gene. Data obtained from transfection experiments and footprinting analysis, suggested that the receptor is one of the major trans-factors implicated in the regulation of its own gene. However, interaction of ER with other transcription factors is required for maximal E-2-stimulation.

引用

页码：123 / 133

页数：11

共 53 条

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