Electrochemically Induced Uptake and Release of Iron by Recombinant Horse H-Chain Ferritin Covalently Immobilized on 6-Mercaptohexanoic Acid Modified Gold Electrodes

被引：7
作者：
Scott, Brandon L. [1 ]
Zapien, Donald C. [1 ]
机构：
[1] Slippery Rock Univ, Dept Chem, Slippery Rock, PA 16057 USA
来源：
ELECTROANALYSIS | 2010年 / 22卷 / 04期
基金：
美国国家科学基金会;
关键词：
Recombinant horse H-chain ferritin; Immobilization; Gold; Self-assembly; Iron uptake; Iron release; CYTOCHROME-C; IONIC-STRENGTH; MONOLAYER; PROTEIN; QUANTITATION; VOLTAMMETRY; TOPOGRAPHY; SPLEEN;
D O I：
10.1002/elan.200900390
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Ferritin composed entirely of the H-chain subunit (rHF) was studied to determine whether electrochemical potential alone could induce rHF to take LIP or release iron. Horse rHF was expressed in E. coli, purified, and covalently immobilized on a self-assembled monolayer of 6-mercaptohexanoic acid on gold electrodes. Cyclic voltammetry was used to show that iron was released from rHF following electrochemical reduction, and iron uptake was induced by applying an oxidizing potential to rHF in the presence of ferrous ion.
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页码：379 / 383
页数：5
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