Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state

被引：34

作者：

Bernardo, Andreia S. ^{[1
,2
]}

Jouneau, Alice ^{[3
]}

Marks, Hendrik ^{[5
]}

Kensche, Philip ^{[4
]}

Kobolak, Julianna ^{[6
]}

Freude, Kristine ^{[7
]}

Hall, Vanessa ^{[7
]}

Feher, Anita ^{[6
]}

Polgar, Zsuzsanna ^{[6
]}

Sartori, Chiara ^{[8
,9
]}

Bock, Istvan ^{[6
]}

Louet, Claire ^{[3
]}

Faial, Tiago ^{[1
]}

Kerstens, Hindrik H. D. ^{[5
]}

Bouissou, Camille ^{[2
]}

Parsonage, Gregory ^{[1
,2
]}

Mashayekhi, Kaveh ^{[6
,7
]}

Smith, James C. ^{[2
]}

Lazzari, Giovanna ^{[8
]}

Hyttel, Poul ^{[7
]}

Stunnenberg, Hendrik G. ^{[5
]}

Huynen, Martijn ^{[4
]}

Pedersen, Roger A. ^{[1
]}

Dinnyes, Andras ^{[6
,10
,11
,12
]}

机构：

[1] Univ Cambridge, Anne McLaren Lab Regenerat Med, Cambridge CB2 0SZ, England

[2] Francis Crick Inst, Dev Biol Dept, 1 Midland Rd, London NW1 1AT, England

[3] Univ Paris Saclay, ENVA, INRA, UMR BDR, F-78350 Jouy En Josas, France

[4] Radboud Univ Nijmegen, Med Ctr, Radboud Inst Mol Life Sci, Ctr Mol & Biomol Informat, POB 9101, NL-6500 HB Nijmegen, Netherlands

[5] Radboud Univ Nijmegen, RIMLS, Fac Sci, Dept Mol Biol, NL-6500 HB Nijmegen, Netherlands

[6] BioTalentum Ltd, H-2100 Godollo, Hungary

[7] Univ Copenhagen, Fac Hlth & Med Sci, Dept Vet Clin & Anim Sci, Groennegaardsvej 7, DK-1870 Frederiksberg C, Denmark

[8] Avantea, Lab Reprod Technol, I-26100 Cremona, Italy

[9] Univ Lausanne, Dept Physiol, CH-1005 Lausanne, Switzerland

[10] Szent Istvan Univ, Mol Anim Biotechnol Lab, H-2100 Godollo, Hungary

[11] Univ Utrecht, Fac Vet Med, Dept Equine Sci, NL-3584 CL Utrecht, Netherlands

[12] Univ Utrecht, Fac Vet Med, Dept Farm Anim Hlth, NL-3584 CL Utrecht, Netherlands

来源：

BIOLOGY OPEN | 2018年 / 7卷 / 08期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

Dusp6; Gjb5; Trip6; Naive; Pluripotency; Primed; EPIBLAST STEM-CELLS; GROUND-STATE; SELF-RENEWAL; IN-VIVO; PRIMITIVE ENDODERM; TRANSCRIPTION; EXPRESSION; DIFFERENTIATION; BLASTOCYST; INHIBITION;

D O I：

10.1242/bio.033282

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

During early mammalian development, transient pools of pluripotent cells emerge that can be immortalised upon stem cell derivation. The pluripotent state, 'naive' or 'primed', depends on the embryonic stage and derivation conditions used. Here we analyse the temporal gene expression patterns of mouse, cattle and porcine embryos at stages that harbour different types of pluripotent cells. We document conserved and divergent traits in gene expression, and identify predictor genes shared across the species that are associated with pluripotent states in vivo and in vitro. Amongst these are the pluripotency-linked genes Klf4 and Lin28b. The novel genes discovered include naive- (Spic, Scpep1 and Gjb5) and primed-associated (Sema6a and Jakmip2) genes as well as naive to primed transition genes (Dusp6 and Trip6). Both Gjb5 and Dusp6 play a role in pluripotency since their knockdown results in differentiation and downregulation of key pluripotency genes. Our interspecies comparison revealed new insights of pluripotency, pluripotent stem cell identity and a new molecular criterion for distinguishing between pluripotent states in various species, including human.

引用

页数：17

共 61 条

[1] Pig Epiblast Stem Cells Depend on Activin/Nodal Signaling for Pluripotency and Self-Renewal [J].