Improving baculovirus recombination

被引：104

作者：

Zhao, YG ^{[1
]}

Chapman, DAG ^{[1
]}

Jones, IM ^{[1
]}

机构：

[1] Univ Reading, Reading RG6 6AL, Berks, England

来源：

NUCLEIC ACIDS RESEARCH | 2003年 / 31卷 / 02期

关键词：

D O I：

10.1093/nar/gng006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant baculoviruses have established themselves as a favoured technology for the high-level expression of recombinant proteins. The construction of recombinant viruses, however, is a time consuming step that restricts consideration of the technology for high throughput developments. Here we use a targeted gene knockout technology to inactivate an essential viral gene that lies adjacent to the locus used for recombination. Viral DNA prepared from the knockout fails to initiate an infection unless rescued by recombination with a baculovirus transfer vector. Modified viral DNA allows 100% recombinant virus formation, obviates the need for further virus purification and offers an efficient means of mass parallel recombinant formation.

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页码：E6 / 6

页数：5

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